LOCUS Exported 2268 bp ds-DNA circular SYN 17-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2268) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 1, 2017 from SnapGene 4.0.5 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2268 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..198 /label=Pnis /note="/ApEinfo_fwdcolor=#9eafd2 /ApEinfo_revcolor=#9eafd2" misc_feature 215..219 /label=BioBrick suffix /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" stem_loop 220..291 /label=E. coli his operon terminator /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" stem_loop 228..262 /label=Stem loop /note="/ApEinfo_fwdcolor=#75c6a9 /ApEinfo_revcolor=#75c6a9" misc_feature complement(355..374) /label=Verification reverse (VR) primer binding site /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" misc_feature complement(459..1073) /label=rep (pMB1) /note="/ApEinfo_fwdcolor=#f8d3a9 /ApEinfo_revcolor=#f8d3a9" misc_feature 474 /label=ORI /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" stem_loop 1240..1345 /label=T0 terminator /note="/ApEinfo_fwdcolor=#d59687 /ApEinfo_revcolor=#d59687" misc_feature complement(1358..2017) /label=Chloramphenicol resistance /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" misc_feature 2131..2150 /label=Verification forward (VF2) primer binding site /note="/ApEinfo_fwdcolor=#85dae9 /ApEinfo_revcolor=#85dae9" stem_loop complement(2207..2210) /label=end of terminator /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" stem_loop complement(2220..2239) /label=Stem loop /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" ORIGIN 1 ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct 61 atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc 121 tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa 181 ttataaggag gcactcacta ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt 241 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc 301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag 481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1201 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa 1261 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg 1321 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat 1381 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 1441 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 1501 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 1561 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 1621 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 1681 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 1741 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa 1801 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 1861 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 1921 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 1981 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 2041 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 2101 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc 2161 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc 2221 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagag //