LOCUS       Exported                2544 bp ds-DNA     circular SYN 13-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    114..133
                     /label=Verification forward (VF2) primer binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     terminator      184..227
                     /label=bacterial terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    230..251
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    252..550
                     /label=pusp45
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     misc_feature    519..524
                     /label=-35
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     misc_feature    539..544
                     /label=-10
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    551..583
                     /label=anti repeat region
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     misc_feature    551..553
                     /label=transcription site
                     /note="/ApEinfo_fwdcolor=#ff9ccd
                     /ApEinfo_revcolor=#ff9ccd"
     misc_RNA        553..631
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     misc_feature    608..638
                     /label=Rho-independent terminator
                     /note="/ApEinfo_fwdcolor=#c7b0e3
                     /ApEinfo_revcolor=#c7b0e3"
     terminator      654..725
                     /label=rrnB T1 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    726..746
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     stem_loop       747..818
                     /label=E. coli his operon terminator
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     misc_feature    complement(882..901)
                     /label=Verification reverse (VR) primer binding site
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    complement(986..1600)
                     /label=rep (pMB1)
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    1001
                     /label=ORI
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     terminator      1770..1864
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1885..2544)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gccgggtata tttactaatc gctggacaag gctttttaca acaattatta
      301 ttgtgaccgc ttttgaagtt tttagtgcaa tcattatgac agcttttgga tttgcccaac
      361 ttcagtttat caaatttgtt gtttaccagt tagcgcctac acttttgctc aatattatct
      421 tagctgtagc cttacaattc cctttagaaa tcttttacag attaaagaaa agtcatgtaa
      481 gatacaatta gaaagtgttt tgtaatcata aagaaatatt aaggtggggt aggaatagta
      541 taatatgttt ttggaaccat tcaaaacagc atagcaagtt aaaataaggc tagtccgtta
      601 tcaacttgaa aaagtggcac cgagtcggtg ctttttttta ctagagtcac actcaaataa
      661 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      721 ctctctacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
      781 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
      841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1741 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1801 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1861 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1921 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1981 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2041 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2101 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2161 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2221 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2281 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2341 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2401 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2461 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2521 atatccagtg atttttttct ccat
//
