LOCUS Exported 2761 bp ds-DNA circular SYN 14-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2761) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 1, 2017 from SnapGene 4.0.5 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2761 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 538..632 /label=lambda t0 terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" CDS complement(653..1312) /label=CmR /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" terminator 1496..1539 /label=bacterial terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" misc_feature complement(1524..1542) /label=SB-prep-2Ea /note="/ApEinfo_fwdcolor=#85dae9 /ApEinfo_revcolor=#85dae9" misc_feature 1542 /label=BioBrick prefix /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" misc_feature 1543..1563 /label=BioBrick prefix /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" misc_feature complement(1543..1549) /label=SB-prep-2Ea /note="/ApEinfo_fwdcolor=#85dae9 /ApEinfo_revcolor=#85dae9" misc_feature 1564..1862 /label=pusp45 /note="/ApEinfo_fwdcolor=#faac61 /ApEinfo_revcolor=#faac61" misc_feature 1831..1836 /label=-35 /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" misc_feature 1851..1856 /label=-10 /note="/ApEinfo_fwdcolor=#ffef86 /ApEinfo_revcolor=#ffef86" misc_feature 1863..2182 /label=array validation /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" misc_feature 1958..1989 /label=Repeat /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" misc_feature 1990..1993 /label=Repeat /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" misc_feature 1994..2023 /label=20 /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" misc_feature 2024..2059 /label=Repeat /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" terminator 2183..2254 /label=rrnB T1 terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" misc_feature 2255..2270 /label=BioBrick suffix /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" misc_feature 2266..2270 /label=SB-prep-3P-1 /note="/ApEinfo_fwdcolor=#f58a5e /ApEinfo_revcolor=#f58a5e" misc_feature 2271..2287 /label=SB-prep-3P-1 /note="/ApEinfo_fwdcolor=#f58a5e /ApEinfo_revcolor=#f58a5e" misc_feature 2271..2275 /label=BioBrick suffix /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" terminator 2276..2333 /label=his operon terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" rep_origin complement(2528..355) /direction=LEFT /label=ori /note="/ApEinfo_fwdcolor=#ffef86 /ApEinfo_revcolor=#ffef86" ORIGIN 1 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 61 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt 541 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc 601 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc 661 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 721 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 781 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 841 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 901 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 961 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 1021 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 1081 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 1141 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 1201 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 1261 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 1321 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 1381 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa 1441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc 1501 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta 1561 gagccgggta tatttactaa tcgctggaca aggcttttta caacaattat tattgtgacc 1621 gcttttgaag tttttagtgc aatcattatg acagcttttg gatttgccca acttcagttt 1681 atcaaatttg ttgtttacca gttagcgcct acacttttgc tcaatattat cttagctgta 1741 gccttacaat tccctttaga aatcttttac agattaaaga aaagtcatgt aagatacaat 1801 tagaaagtgt tttgtaatca taaagaaata ttaaggtggg gtaggaatag tataatatgt 1861 ttaataaatg cagtaataca ggggcttttc aagactgaag tctagctgag acaaatagtg 1921 cgattacgaa attttttaga caaaaatagt ctacgaggtt ttagagctat gctgttttga 1981 atggtcccaa aacatttcaa ccttaatagt agacaacgca ggagttttag agctatgctg 2041 ttttgaatgg tcccaaaaca acattgccga tgataacttg agaaagaggg ttaataccag 2101 cagtcggata ccttcctatt ctttctgtta aagcgttttc atgttataat aggcaaaaga 2161 agagtagtgt gatggaacaa accaaataaa acgaaaggct cagtcgaaag actgggcctt 2221 tcgttttatc tgttgtttgt cggtgaacgc tctctactag tagcggccgc tgcagtccgg 2281 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc 2341 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 2401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 2461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 2521 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 2581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 2641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 2701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 2761 t //