LOCUS       Exported                2761 bp ds-DNA     circular SYN 14-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      184..227
                     /label=bacterial terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    230..251
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    252..550
                     /label=pusp45
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     misc_feature    519..524
                     /label=-35
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     misc_feature    539..544
                     /label=-10
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    646..681
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    682..711
                     /label=21
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    712..747
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     terminator      871..942
                     /label=rrnB T1 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    943..963
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      964..1021
                     /label=his operon terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    complement(1203..1817)
                     /label=rep (pMB1)
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     terminator      1987..2081
                     /label=lambda t0 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     CDS             complement(2102..2761)
                     /label=CmR
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gccgggtata tttactaatc gctggacaag gctttttaca acaattatta
      301 ttgtgaccgc ttttgaagtt tttagtgcaa tcattatgac agcttttgga tttgcccaac
      361 ttcagtttat caaatttgtt gtttaccagt tagcgcctac acttttgctc aatattatct
      421 tagctgtagc cttacaattc cctttagaaa tcttttacag attaaagaaa agtcatgtaa
      481 gatacaatta gaaagtgttt tgtaatcata aagaaatatt aaggtggggt aggaatagta
      541 taatatgttt aataaatgca gtaatacagg ggcttttcaa gactgaagtc tagctgagac
      601 aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt agagctatgc
      661 tgttttgaat ggtcccaaaa cagcagttct gtttaggcgg aagcaatact cgttttagag
      721 ctatgctgtt ttgaatggtc ccaaaacaac attgccgatg ataacttgag aaagagggtt
      781 aataccagca gtcggatacc ttcctattct ttctgttaaa gcgttttcat gttataatag
      841 gcaaaagaag agtagtgtga tggaacaaac caaataaaac gaaaggctca gtcgaaagac
      901 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagta gcggccgctg
      961 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1021 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1081 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1141 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1201 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1261 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1321 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1381 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1441 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1501 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1561 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1621 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1681 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1801 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1861 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1921 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1981 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2041 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2101 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2161 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2221 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2281 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2341 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2401 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2461 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2521 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2581 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2641 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2701 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2761 t
//
