LOCUS Exported 2709 bp ds-DNA circular SYN 14-OCT-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2709) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 1, 2017 from SnapGene 4.0.5 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2709 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 184..227 /label=bacterial terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" misc_feature 230..251 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 252..550 /label=pusp45 /note="/ApEinfo_fwdcolor=#faac61 /ApEinfo_revcolor=#faac61" misc_feature 519..524 /label=-35 /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" misc_feature 539..544 /label=-10 /note="/ApEinfo_fwdcolor=#ffef86 /ApEinfo_revcolor=#ffef86" misc_feature 646..681 /label=Repeat /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" misc_feature 683..688 /label=BsaI Cutsite /label=RE /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" misc_feature 705..710 /label=BsaI Cutsite /label=RE /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" terminator 819..890 /label=rrnB T1 terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" misc_feature 891..911 /label=BioBrick suffix /note="universal suffix for all parts" terminator 912..969 /label=his operon terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" misc_feature complement(1151..1765) /label=rep (pMB1) /note="/ApEinfo_fwdcolor=#f8d3a9 /ApEinfo_revcolor=#f8d3a9" terminator 1935..2029 /label=lambda t0 terminator /note="/ApEinfo_fwdcolor=#c6c9d1 /ApEinfo_revcolor=#c6c9d1" CDS complement(2050..2709) /label=CmR /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" ORIGIN 1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg 121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc 241 cgcttctaga gccgggtata tttactaatc gctggacaag gctttttaca acaattatta 301 ttgtgaccgc ttttgaagtt tttagtgcaa tcattatgac agcttttgga tttgcccaac 361 ttcagtttat caaatttgtt gtttaccagt tagcgcctac acttttgctc aatattatct 421 tagctgtagc cttacaattc cctttagaaa tcttttacag attaaagaaa agtcatgtaa 481 gatacaatta gaaagtgttt tgtaatcata aagaaatatt aaggtggggt aggaatagta 541 taatatgttt aataaatgca gtaatacagg ggcttttcaa gactgaagtc tagctgagac 601 aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt agagctatgc 661 tgttttgaat ggtcccaaaa ctgagaccaa cattgccgat gataggtctc gacttgagaa 721 agagggttaa taccagcagt cggatacctt cctattcttt ctgttaaagc gttttcatgt 781 tataataggc aaaagaagag tagtgtgatg gaacaaacca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagtagc 901 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa 961 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc 1021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 1081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 1141 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 1201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 1621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 1861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 1921 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac 1981 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc 2041 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat 2101 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag 2161 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc 2221 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa 2281 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac 2341 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga 2401 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat 2461 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc 2521 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa 2581 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc 2641 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt 2701 tttctccat //