LOCUS Exported 4033 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0049max_producer_lasI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4033) AUTHORS Daniel Gerngross TITLE piG0049max JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4033 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 2..96 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" promoter 150..184 /note="const. promoter (J23100)" CDS 218..826 /codon_start=1 /product="LasI (no LVA)" /note="lasI (C0178)" /translation="MIVQIGRREEFDKKLLGEMHKLRAQVFKERKGWDVSVIDEMEIDG YDALSPYYMLIQEDTPEAQVFGCWRIFDTTGPYMLKNTFPELLHGKEAPCSPHIWELSR FAINSGQKGSLGFSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFG PHLKIGIERAVALRIELNAKTQIALYGGVLVEQRLAVS*" terminator 838..924 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 1039..1810 /note="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1811..2473 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /note="pBBR1 Rep" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" oriT 2484..2592 /note="incP origin of transfer" CDS complement(2738..3928) /note="TetR" ORIGIN 1 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga 61 gttctgaggt cattactgga tctatcaaca ggagtccaag actagtcgcc agggttttcc 121 cagtcacgac gcggccgcaa gcttgcatgt tgacggctag ctcagtccta ggtacagtgc 181 tagcgcggga agaacccgca acaataagga gggtaatatg atcgttcaga tcggtcgtcg 241 tgaagagttc gacaaaaaac tgctgggtga aatgcacaaa ctgcgtgctc aggttttcaa 301 agaacgtaaa ggttgggacg tttccgttat cgacgaaatg gaaatcgacg gttacgacgc 361 tctgtccccg tactacatgc tgatccagga agacaccccg gaagctcagg ttttcggttg 421 ctggcgtatc ttcgacacca ccggtccgta catgctgaaa aacaccttcc cggaactgct 481 gcacggtaaa gaagctccgt gctccccgca catctgggaa ctgtcccgtt tcgctatcaa 541 ctccggtcag aaaggttccc tgggtttctc cgactgcacc ctggaagcta tgcgtgctct 601 ggctcgttac tccttgcaga acgacatcca gaccctggtt accgttacca ccgttggtgt 661 tgaaaaaatg atgatccgtg ctggtctgga cgtttcccgt ttcggtccgc acctgaaaat 721 cggtatcgaa cgtgctgttg ctctgcgtat cgaactgaac gctaaaaccc agatcgctct 781 gtacggtggt gttctggttg aacagcgtct ggctgtttcc taataaatta aaggcatcaa 841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 901 aacgctctcc tgagtaggac aaatccgccg ccctagacag ctgggcgcgc cccccctacg 961 ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct cccttgccag cccgtggata 1021 tgtggacgat ggccgcgagc ggccaccggc tggctcgctt cgctcggccc gtggacaacc 1081 ctgctggaca agctgatgga caggctgcgc ctgcccacga gcttgaccac agggattgcc 1141 caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg cctgcggcct 1201 tgccccatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc 1261 ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca 1321 gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag 1381 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg 1441 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg 1501 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc 1561 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa 1621 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt 1681 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc 1741 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc cctaccgcat 1801 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg 1861 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa 1921 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt 1981 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata 2041 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg 2101 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt 2161 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct 2221 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc 2281 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct 2341 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc 2401 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtaacgct 2461 gccgcgccgg tagggccggc ctacggccag cctcgcagag caggattccc gttgagcacc 2521 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca 2581 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg 2641 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc 2701 ccgaagcagg gttatgcagc ggaaaaggac aattgtctca ggtcgaggtg gcccggctcc 2761 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg 2821 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag 2881 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt 2941 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga 3001 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc 3061 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg 3121 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca 3181 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg 3241 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gccacaatgg 3301 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggac 3361 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt 3421 tgagcaccgc cgccgcaagg aatggtgcgt gcagggagat ggcgcccaac agtcccccgg 3481 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag 3541 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc 3601 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca 3661 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa 3721 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg 3781 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc 3841 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga 3901 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact 3961 gtgataaact accgcattac agtttatcga tgataagctg tcaaatttaa atcgtaatta 4021 ttggggaccc ctg //