LOCUS Exported 3851 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI- SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3851) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3851 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="R2oDNA spacer" /note="R2oDNA 70bp spacer 13" terminator 203..230 /note="T7Te" /note="phage T7 early transcription terminator" misc_feature complement(238..312) /note="taR12y" promoter complement(313..367) /note="lux rR (R0062)" misc_feature 368..437 /note="spacer (B0040)" promoter 438..492 /note="lux rR (R0062)" 3'UTR 493..548 /note="crR12y" RBS 531..542 /note="RBS (B0034)" CDS 549..1274 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1277..1393 /note="T1" terminator 1286..1372 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1400..1419 /note="AB179" primer_bind 1585..1604 /note="AB5" rep_origin 1738..2565 /direction=RIGHT /note="p15A" rep_origin 1986..1988 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1989..2533) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2637..2656 /note="AB180" oriT 2667..2775 /note="incP origin of transfer" misc_feature 2747 /note="C->T no real mutation, just for testing" CDS complement(2931..3746) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg 181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct 241 agctagagat atatggtagt agtaagttaa ttttcattaa ccaccactac caatcacctc 301 ctggatttag cttttattcg actataacaa accattttct tgcgtaaacc tgtacgatcc 361 tacaggtagg ttctgttaag taactgaacc caatgtcgtt agtgacgctt acctcttaag 421 aggtcactga cctaacaacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 481 tagtcgaata aagaattaat tctaccattc acctcttgga tttagctatt aaagaggaga 541 aaggtaccat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 601 tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta 661 caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc 721 caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca 781 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta 841 tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata 901 cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg 961 gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa 1021 agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac 1081 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 1141 accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca 1201 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca 1261 aaaggcctta ataaattaaa ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1321 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc 1381 ctagacagct gggcgcgcca aatacgcccg gtagtgatct tatttcatta tggtgaaagt 1441 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 1501 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 1561 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg 1621 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact 1681 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac 1741 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa 1801 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct 1861 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg 1921 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca 1981 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat 2041 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc 2101 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc 2161 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac 2221 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt 2281 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt 2341 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg 2401 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt 2461 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc 2521 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagc atgagcggat 2581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 2641 aagtgccacc tgctagggcc ggcctacggc cagcctcgca gagcaggatt cccgttgagc 2701 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgtggg tgggcctact 2761 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt 2821 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg 2881 accccgaagc agggttatgc agcggaaaag gacccttgtc caattaatta ttagaaaaat 2941 tcatccagca tcagatgaaa ttgcagtttg ttcatatccg gattatcaat gccatatttc 3001 tgaaacagac gtttttgcag gctcgggcta aattcgccca ggcagttcca cagaatggcc 3061 agatcctgat aacgatccgc aatgcccaca cggcccacat caatgcagcc aatcagtttg 3121 ccttcatcga aaatcaggtt atccaggcta aaatcgccgt gggtcaccac gctatccggg 3181 ctaaacggca gcagtttatg catttctttc cacacctgtt ccaccggcca gccgttacgt 3241 tcatcatcaa aatcgctcgc atccaccagg ccgttgttca tacggctctg cgcctgggcc 3301 agacgaaaca cacgatcgct gttaaacggg cagttgcaca ccggaatgct atgcagacga 3361 cgcagaaaca cggccagcgc atccacaatg ttttcgccgc tatccggata ttcttccagc 3421 acctgaaacg cggttttgcc cggaatcgcg gtggtcagca gccacgcatc atccggggtg 3481 cgaataaaat gtttaatggt cggcagcggc ataaattcgg tcagccagtt cagacgcacc 3541 atttcatcgg tcacatcgtt cgccacgctg cctttgccat gtttcagaaa cagttccggc 3601 gcatccggtt tgccatacag acgataaatg gtcgcgccgc tctgacccac gttatcacgc 3661 gcccatttat agccatacag atccgcatcc atgttgctgt tcagacgcgg acggctacag 3721 ctcgtttcac gctgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac 3781 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga 3841 gacacaaatt t //