LOCUS Exported 4013 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0051max_producer_rhlI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4013) AUTHORS Daniel Gerngross TITLE piG0051max JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4013 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..84 /note="const. promoter (J23100)" RBS 87..91 /note="RBS (B0034)" CDS 98..700 /codon_start=1 /product="RhlI-LVA-Barcode" /note="rhlI (C0070)" /translation="MIELLSESLEGLSAAMIAELGRYRHQVFIEKLGWDVVSTSRVRDQ EFDQFDHPQTRYIVAMSRQGICGCARLLPTTDAYLLKDVFAYLCSETPPSDPSVWELSR YAASAADDPQLAMKIFWSSLQCAWYLGASSVVAVTTTAMERYFVRNGVILQRLGPPQKV KGETLVAISFPAYQERGLEMLLRYHPEWLQGVPLSMAV" terminator 718..804 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 919..1690 /note="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1691..2353 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /note="pBBR1 Rep" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" oriT 2364..2472 /note="incP origin of transfer" CDS complement(2618..3808) /note="TetR" terminator 3915..4009 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" ORIGIN 1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag 61 ctcagtccta ggtacagtgc tagctaagga ggtatcaatg atcgaactgc tgtccgaatc 121 cctggaaggt ctgtccgctg ctatgatcgc tgaactgggt cgttaccgtc accaggtttt 181 catcgaaaaa ctgggttggg acgttgtttc cacctcccgt gttcgtgacc aggagttcga 241 ccagttcgac cacccgcaga cccgttacat cgttgctatg tcccgtcagg gtatctgcgg 301 ttgcgctcgt ctgctgccga ccaccgacgc ttacctgctg aaagacgttt tcgcttacct 361 gtgctccgaa accccgccgt ccgacccgtc cgtttgggaa ctgtcccgtt acgctgcttc 421 cgctgctgac gacccgcagc tggctatgaa aatcttctgg tcctccctcc agtgcgcttg 481 gtacctgggt gcttcctccg ttgttgctgt taccaccacc gctatggaac gttacttcgt 541 tcgtaacggt gttatcctcc agcgtctggg tccgccgcag aaagttaaag gtgaaaccct 601 ggttgctatc tccttcccgg cttaccagga acgtggtctg gaaatgctgc tgcgttacca 661 cccggaatgg ctccagggtg ttccgctgtc catggctgtt taataaatta aaggcatcaa 721 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 781 aacgctctcc tgagtaggac aaatccgccg ccctagacag ctgggcgcgc cccccctacg 841 ggcttgctct ccgggcttcg ccctgcgcgg tcgctgcgct cccttgccag cccgtggata 901 tgtggacgat ggccgcgagc ggccaccggc tggctcgctt cgctcggccc gtggacaacc 961 ctgctggaca agctgatgga caggctgcgc ctgcccacga gcttgaccac agggattgcc 1021 caccggctac ccagccttcg accacatacc caccggctcc aactgcgcgg cctgcggcct 1081 tgccccatca atttttttaa ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc 1141 ttcgcttgcc ggttggacac caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca 1201 gcggcttggc cttgacgcgc ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag 1261 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg 1321 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg 1381 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc 1441 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa 1501 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt 1561 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc 1621 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc cctaccgcat 1681 ggagataagc atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg 1741 tcactgggtg caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa 1801 acccacggcg gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt 1861 ggtggtcagc cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata 1921 cgcagtcaag gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg 1981 caccgtgtcg gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt 2041 gcgcctgtcg gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct 2101 gttggggcat ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc 2161 gaccggcccc ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct 2221 gccagccttg accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc 2281 cgatgagccg tgttttctgg acgatggcga gccgttggag ccgccgacac gggtaacgct 2341 gccgcgccgg tagggccggc ctacggccag cctcgcagag caggattccc gttgagcacc 2401 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca 2461 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg 2521 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc 2581 ccgaagcagg gttatgcagc ggaaaaggac aattgtctca ggtcgaggtg gcccggctcc 2641 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg 2701 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag 2761 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt 2821 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga 2881 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc 2941 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg 3001 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca 3061 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg 3121 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gccacaatgg 3181 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggac 3241 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt 3301 tgagcaccgc cgccgcaagg aatggtgcgt gcagggagat ggcgcccaac agtcccccgg 3361 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag 3421 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc 3481 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca 3541 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa 3601 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg 3661 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc 3721 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga 3781 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact 3841 gtgataaact accgcattac agtttatcga tgataagctg tcaaatttaa atcgtaatta 3901 ttggggaccc ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac 3961 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aag //