LOCUS Exported 4191 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0099_pluxstRBS-sfGFP-stRBS-luxI SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 4191) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4191 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="spacer (B0040)" promoter 197..251 /note="lux rR (R0062)" RBS 260..271 /note="RBS (B0034)" CDS 278..1003 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" RBS 1012..1023 /note="RBS (B0034)" CDS 1030..1614 /codon_start=1 /note="luxI (C0161)" /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*" terminator 1626..1712 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1706..1733 /note="T1" primer_bind 1740..1759 /note="AB179" primer_bind 1925..1944 /note="AB5" rep_origin 2078..2905 /direction=RIGHT /note="p15A" rep_origin 2326..2328 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(2329..2873) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2977..2996 /note="AB180" oriT 3007..3115 /note="incP origin of transfer" CDS complement(3271..4086) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga 181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 241 agtcgaataa atactagaga aagaggagaa atactagatg agcaaaggag aagaactttt 301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 361 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg 421 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt 481 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat 541 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac 601 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat 661 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca 721 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg 781 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat 841 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc 901 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg 961 gattacacat ggcatggatg agctctacaa aaggccttaa taatactagt gaaagaggag 1021 aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag 1081 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta 1141 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat 1201 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat 1261 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct 1321 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct 1381 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt 1441 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa 1501 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt 1561 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatta ataaattaaa 1621 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1681 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacagct gggcgcgcca 1741 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga 1801 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca 1861 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg 1921 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt 1981 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg 2041 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact 2101 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc 2161 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg 2221 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg 2281 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct 2341 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac 2401 aggactataa agataccagg cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct 2461 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct 2521 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt 2581 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca 2641 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt 2701 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc 2761 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg 2821 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc 2881 atcttattaa tcagataaaa tatttctagc atgagcggat acatatttga atgtatttag 2941 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgctagggcc 3001 ggcctacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg 3061 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg 3121 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg 3181 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc 3241 agcggaaaag gacccttgtc caattaatta ttagaaaaat tcatccagca tcagatgaaa 3301 ttgcagtttg ttcatatccg gattatcaat gccatatttc tgaaacagac gtttttgcag 3361 gctcgggcta aattcgccca ggcagttcca cagaatggcc agatcctgat aacgatccgc 3421 aatgcccaca cggcccacat caatgcagcc aatcagtttg ccttcatcga aaatcaggtt 3481 atccaggcta aaatcgccgt gggtcaccac gctatccggg ctaaacggca gcagtttatg 3541 catttctttc cacacctgtt ccaccggcca gccgttacgt tcatcatcaa aatcgctcgc 3601 atccaccagg ccgttgttca tacggctctg cgcctgggcc agacgaaaca cacgatcgct 3661 gttaaacggg cagttgcaca ccggaatgct atgcagacga cgcagaaaca cggccagcgc 3721 atccacaatg ttttcgccgc tatccggata ttcttccagc acctgaaacg cggttttgcc 3781 cggaatcgcg gtggtcagca gccacgcatc atccggggtg cgaataaaat gtttaatggt 3841 cggcagcggc ataaattcgg tcagccagtt cagacgcacc atttcatcgg tcacatcgtt 3901 cgccacgctg cctttgccat gtttcagaaa cagttccggc gcatccggtt tgccatacag 3961 acgataaatg gtcgcgccgc tctgacccac gttatcacgc gcccatttat agccatacag 4021 atccgcatcc atgttgctgt tcagacgcgg acggctacag ctcgtttcac gctgaatatg 4081 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga 4141 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaaatt t //