LOCUS Exported 4008 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0050max_producer_luxI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4008) AUTHORS Daniel Gerngross TITLE piG0050max JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4008 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..84 /note="const. promoter (J23100)" RBS 105..109 /note="RBS (B0034)" CDS 117..701 /codon_start=1 /note="luxI (C0161)" /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*" terminator 713..799 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 914..1685 /note="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1686..2348 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /note="pBBR1 Rep" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" oriT 2359..2467 /note="incP origin of transfer" CDS complement(2613..3803) /note="TetR" terminator 3910..4004 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" ORIGIN 1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag 61 ctcagtccta ggtacagtgc tagcagcgaa acacgcatat tttaaggagg tactacatga 121 ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc 181 taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata 241 accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata 301 ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa 361 gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat 421 taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta 481 caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg 541 taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc 601 gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc 661 ctattaatga acagtttaaa aaagcagtct taaattaata aattaaaggc atcaaataaa 721 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 781 tctcctgagt aggacaaatc cgccgcccta gacagctggg cgcgcccccc ctacgggctt 841 gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt ggatatgtgg 901 acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga caaccctgct 961 ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga ttgcccaccg 1021 gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc ggccttgccc 1081 catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg cgcgcttcgc 1141 ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc gcgcagcggc 1201 ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt cgaagggcga 1261 agcccgcccg cctgcccccc gagcctcacg gcggcgagtg cgggggttcc aagggggcag 1321 cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa caagccccgg aggggccact 1381 ttttgccgga gggggagccg cgccgaaggc gtgggggaac cccgcagggg tgcccttctt 1441 tgggcaccaa agaactagat atagggcgaa atgcgaaaga cttaaaaatc aacaacttaa 1501 aaaagggggg tacgcaacag ctcattgcgg caccccccgc aatagctcat tgcgtaggtt 1561 aaagaaaatc tgtaattgac tgccactttt acgcaacgca taattgttgt cgcgctgccg 1621 aaaagttgca gctgattgcg catggtgccg caaccgtgcg gcacccctac cgcatggaga 1681 taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag cccggtcact 1741 gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca 1801 cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg 1861 tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc caatacgcag 1921 tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg 1981 tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc 2041 tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa tcgctgttgg 2101 ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg 2161 gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca gacctgccag 2221 ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg atgcccgatg 2281 agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggta acgctgccgc 2341 gccggtaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga gcaccgccag 2401 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat 2461 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa 2521 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa 2581 gcagggttat gcagcggaaa aggacaattg tctcaggtcg aggtggcccg gctccatgca 2641 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac 2701 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc 2761 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct 2821 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga 2881 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc 2941 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga 3001 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg 3061 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc 3121 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggccac aatggtcatg 3181 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggacggcgc 3241 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc 3301 accgccgccg caaggaatgg tgcgtgcagg gagatggcgc ccaacagtcc cccggccacg 3361 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga 3421 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg 3481 atgccggcca cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc 3541 gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg 3601 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc 3661 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc 3721 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg 3781 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat 3841 aaactaccgc attacagttt atcgatgata agctgtcaaa tttaaatcgt aattattggg 3901 gacccctgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc 3961 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaag //