LOCUS Exported 4026 bp ds-DNA circular SYN 10-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0049_producer_lasI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4026) AUTHORS Daniel Gerngross TITLE piG0049 JOURNAL Exported 10 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4026 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 50..84 /note="const. promoter (J23100)" RBS 93..104 /note="RBS (B0034)" CDS 111..719 /codon_start=1 /product="LasI (no LVA)" /note="lasI (C0178)" /translation="MIVQIGRREEFDKKLLGEMHKLRAQVFKERKGWDVSVIDEMEIDG YDALSPYYMLIQEDTPEAQVFGCWRIFDTTGPYMLKNTFPELLHGKEAPCSPHIWELSR FAINSGQKGSLGFSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFG PHLKIGIERAVALRIELNAKTQIALYGGVLVEQRLAVS*" terminator 731..817 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 932..1703 /note="pBBR1 oriV" /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1704..2366 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /note="pBBR1 Rep" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" oriT 2377..2485 /note="incP origin of transfer" CDS complement(2631..3821) /note="TetR" terminator 3928..4022 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" ORIGIN 1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag 61 ctcagtccta ggtacagtgc tagctactag tgaaagagga gaaatactag atgatcgttc 121 agatcggtcg tcgtgaagag ttcgacaaaa aactgctggg tgaaatgcac aaactgcgtg 181 ctcaggtttt caaagaacgt aaaggttggg acgtttccgt tatcgacgaa atggaaatcg 241 acggttacga cgctctgtcc ccgtactaca tgctgatcca ggaagacacc ccggaagctc 301 aggttttcgg ttgctggcgt atcttcgaca ccaccggtcc gtacatgctg aaaaacacct 361 tcccggaact gctgcacggt aaagaagctc cgtgctcccc gcacatctgg gaactgtccc 421 gtttcgctat caactccggt cagaaaggtt ccctgggttt ctccgactgc accctggaag 481 ctatgcgtgc tctggctcgt tactccttgc agaacgacat ccagaccctg gttaccgtta 541 ccaccgttgg tgttgaaaaa atgatgatcc gtgctggtct ggacgtttcc cgtttcggtc 601 cgcacctgaa aatcggtatc gaacgtgctg ttgctctgcg tatcgaactg aacgctaaaa 661 cccagatcgc tctgtacggt ggtgttctgg ttgaacagcg tctggctgtt tcctaataaa 721 ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 781 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cagctgggcg 841 cgccccccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc gctcccttgc 901 cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg cttcgctcgg 961 cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca cgagcttgac 1021 cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc tccaactgcg 1081 cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag cctccggcct 1141 gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc aaggctcgcg 1201 cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc tatgcgagtg 1261 ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc ggcgagtgcg 1321 ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag tcgatcaaca 1381 agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt gggggaaccc 1441 cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat gcgaaagact 1501 taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca ccccccgcaa 1561 tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac gcaacgcata 1621 attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca accgtgcggc 1681 acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc attcaagcca 1741 agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg tgggccgggc 1801 ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg cagatgggcc 1861 accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga cgttctttgc 1921 ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc gtcgtgaagc 1981 tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg tggggccagc 2041 cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat cacgacgacc 2101 aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg tatccgggcg 2161 agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc attccgggca 2221 tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc gggcagcagc 2281 gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg gagccgccga 2341 cacgggtaac gctgccgcgc cggtagggcc ggcctacggc cagcctcgca gagcaggatt 2401 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg 2461 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac 2521 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat 2581 cgctataatg accccgaagc agggttatgc agcggaaaag gacaattgtc tcaggtcgag 2641 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg 2701 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg 2761 atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt 2821 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg 2881 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc 2941 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg 3001 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat 3061 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg 3121 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt 3181 gcggccacaa tggtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc 3241 aagggcatcg gacggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt 3301 aggttgaggc cgttgagcac cgccgccgca aggaatggtg cgtgcaggga gatggcgccc 3361 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc 3421 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc 3481 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg 3541 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact 3601 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc 3661 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata 3721 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg 3781 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt 3841 tagcaattta actgtgataa actaccgcat tacagtttat cgatgataag ctgtcaaatt 3901 taaatcgtaa ttattgggga cccctggatt ctcaccaata aaaaacgccc ggcggcaacc 3961 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag 4021 tccaag //