LOCUS Exported 3606 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0062_sensor_pluxRcr12yRBS-sfGFP SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3606) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3606 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="spacer (B0040)" promoter 197..251 /note="lux rR (R0062)" 3'UTR 252..303 /note="crR12y" RBS 286..297 /note="RBS (B0034)" CDS 304..1029 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1032..1148 /note="T1" terminator 1041..1127 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1155..1174 /note="AB179" primer_bind 1340..1359 /note="AB5" rep_origin 1493..2320 /direction=RIGHT /note="p15A" rep_origin 1741..1743 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1744..2288) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2392..2411 /note="AB180" oriT 2422..2530 /note="incP origin of transfer" CDS complement(2686..3501) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga 181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 241 agtcgaataa agaattctac cattcacctc ttggatttag ctattaaaga ggagaaaggt 301 accatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 361 ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac 421 ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca 481 cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa 541 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct 601 ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt 661 gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac 721 aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat 781 ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca 841 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 901 tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc 961 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaagg 1021 ccttaataaa ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 1081 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga 1141 cagctgggcg cgccaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa 1201 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta 1261 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat 1321 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt 1381 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg 1441 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct 1501 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg 1561 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg 1621 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag 1681 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc 1741 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg 1801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccctggcgg ctccctcgtg 1861 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg 1921 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat 1981 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 2041 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg 2101 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact 2161 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa 2221 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac 2281 gatctcaaga agatcatctt attaatcaga taaaatattt ctagcatgag cggatacata 2341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 2401 ccacctgcta gggccggcct acggccagcc tcgcagagca ggattcccgt tgagcaccgc 2461 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc 2521 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca 2581 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc 2641 gaagcagggt tatgcagcgg aaaaggaccc ttgtccaatt aattattaga aaaattcatc 2701 cagcatcaga tgaaattgca gtttgttcat atccggatta tcaatgccat atttctgaaa 2761 cagacgtttt tgcaggctcg ggctaaattc gcccaggcag ttccacagaa tggccagatc 2821 ctgataacga tccgcaatgc ccacacggcc cacatcaatg cagccaatca gtttgccttc 2881 atcgaaaatc aggttatcca ggctaaaatc gccgtgggtc accacgctat ccgggctaaa 2941 cggcagcagt ttatgcattt ctttccacac ctgttccacc ggccagccgt tacgttcatc 3001 atcaaaatcg ctcgcatcca ccaggccgtt gttcatacgg ctctgcgcct gggccagacg 3061 aaacacacga tcgctgttaa acgggcagtt gcacaccgga atgctatgca gacgacgcag 3121 aaacacggcc agcgcatcca caatgttttc gccgctatcc ggatattctt ccagcacctg 3181 aaacgcggtt ttgcccggaa tcgcggtggt cagcagccac gcatcatccg gggtgcgaat 3241 aaaatgttta atggtcggca gcggcataaa ttcggtcagc cagttcagac gcaccatttc 3301 atcggtcaca tcgttcgcca cgctgccttt gccatgtttc agaaacagtt ccggcgcatc 3361 cggtttgcca tacagacgat aaatggtcgc gccgctctga cccacgttat cacgcgccca 3421 tttatagcca tacagatccg catccatgtt gctgttcaga cgcggacggc tacagctcgt 3481 ttcacgctga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt 3541 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac 3601 aaattt //