LOCUS Exported 3835 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0066_sensor_prhlRRR12-sfGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3835) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3835 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="R2oDNA spacer" /note="R2oDNA 70bp spacer 29" terminator 203..230 /note="T7Te terminator" /note="phage T7 early transcription terminator" misc_feature complement(238..308) /note="taR12" promoter complement(309..359) /note="P(Rhl) (I14017)" misc_feature 360..429 /note="spacer (B0040)" promoter 430..480 /note="P(Rhl) (I14017)" misc_feature 481..532 /note="crR12" RBS 515..526 /note="RBS (B0034)" CDS 533..1258 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1261..1377 /note="T1" terminator 1270..1356 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1384..1403 /note="AB179" primer_bind 1569..1588 /note="AB5" rep_origin 1722..2549 /direction=RIGHT /note="p15A" rep_origin 1970..1972 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1973..2517) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2621..2640 /note="AB180" oriT 2651..2759 /note="incP origin of transfer" misc_feature 2731 /note="C->T just for test, no real mutation" CDS complement(2915..3730) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagatgtg agtcctgtta cgcccgtgtt ttccagtaga ataatagtga caatcgtttc 181 agataagcaa ccaatatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct 241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg 301 atttgggttt cgactataac aaaccatttt cttgcgtaag cctaccaatt catgcaggaa 361 ggttctgtta agtaactgaa cccaatgtcg ttagtgacgc ttacctctta agaggtcact 421 gacctaacat cctgcatgaa ttggtaggct tacgcaagaa aatggtttgt tatagtcgaa 481 gaattctacc attcacctct tggatttggg tattaaagag gagaaaggta ccatgagcaa 541 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa 601 tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac 661 ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac 721 tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt 781 tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga 841 cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat 901 cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgagta 961 caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc 1021 taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag accattatca 1081 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac 1141 acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt 1201 tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaaaggc cttaataaat 1261 taaaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1321 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac agctgggcgc 1381 gccaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 1441 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac 1501 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag 1561 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc 1621 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta 1681 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg 1741 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg 1801 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc 1861 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa 1921 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata 1981 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc 2041 cgacaggact ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt 2101 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac 2161 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc 2221 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa 2281 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg 2341 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca 2401 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc 2461 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa 2521 gatcatctta ttaatcagat aaaatatttc tagcatgagc ggatacatat ttgaatgtat 2581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgctag 2641 ggccggccta cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat 2701 aagggacagt gaagaaggaa cacccgctcg tgggtgggcc tacttcacct atcctgcccg 2761 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat 2821 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt 2881 atgcagcgga aaaggaccct tgtccaatta attattagaa aaattcatcc agcatcagat 2941 gaaattgcag tttgttcata tccggattat caatgccata tttctgaaac agacgttttt 3001 gcaggctcgg gctaaattcg cccaggcagt tccacagaat ggccagatcc tgataacgat 3061 ccgcaatgcc cacacggccc acatcaatgc agccaatcag tttgccttca tcgaaaatca 3121 ggttatccag gctaaaatcg ccgtgggtca ccacgctatc cgggctaaac ggcagcagtt 3181 tatgcatttc tttccacacc tgttccaccg gccagccgtt acgttcatca tcaaaatcgc 3241 tcgcatccac caggccgttg ttcatacggc tctgcgcctg ggccagacga aacacacgat 3301 cgctgttaaa cgggcagttg cacaccggaa tgctatgcag acgacgcaga aacacggcca 3361 gcgcatccac aatgttttcg ccgctatccg gatattcttc cagcacctga aacgcggttt 3421 tgcccggaat cgcggtggtc agcagccacg catcatccgg ggtgcgaata aaatgtttaa 3481 tggtcggcag cggcataaat tcggtcagcc agttcagacg caccatttca tcggtcacat 3541 cgttcgccac gctgcctttg ccatgtttca gaaacagttc cggcgcatcc ggtttgccat 3601 acagacgata aatggtcgcg ccgctctgac ccacgttatc acgcgcccat ttatagccat 3661 acagatccgc atccatgttg ctgttcagac gcggacggct acagctcgtt tcacgctgaa 3721 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg 3781 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca aattt //