LOCUS Exported 3580 bp ds-DNA circular SYN 17-OCT-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS piG0059_sensor_pluxRstRBS-sfGFP SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3580) AUTHORS Daniel Gerngross TITLE Direct Submission JOURNAL Exported 17 Oct 2014 from SnapGene 2.5.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3580 /organism="recombinant plasmid" /mol_type="other DNA" terminator 1..126 /note="T0" terminator 27..121 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" misc_feature 127..196 /note="spacer (B0040)" promoter 197..251 /note="lux rR (R0062)" RBS 260..271 /note="RBS (B0034)" CDS 278..1003 /codon_start=1 /note="sfGFP" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYKRP*" terminator 1006..1122 /note="T1" terminator 1015..1101 /gene="Escherichia coli rrnB" /note="T1" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1129..1148 /note="AB179" primer_bind 1314..1333 /note="AB5" rep_origin 1467..2294 /direction=RIGHT /note="p15A" rep_origin 1715..1717 /direction=RIGHT /note="P15 ori from pACYC184" rep_origin complement(1718..2262) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." primer_bind 2366..2385 /note="AB180" oriT 2396..2504 /note="incP origin of transfer" CDS complement(2660..3475) /note="KamR" ORIGIN 1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg 61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga 181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 241 agtcgaataa atactagaga aagaggagaa atactagatg agcaaaggag aagaactttt 301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc 361 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg 421 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt 481 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat 541 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac 601 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat 661 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca 721 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg 781 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat 841 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc 901 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg 961 gattacacat ggcatggatg agctctacaa aaggccttaa taaattaaag gcatcaaata 1021 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1081 gctctcctga gtaggacaaa tccgccgccc tagacagctg ggcgcgccaa atacgcccgg 1141 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca 1201 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta 1261 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc 1321 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt 1381 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg 1441 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt 1501 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg 1561 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg 1621 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata 1681 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg 1741 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa 1801 gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt 1861 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt 1921 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc 1981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac 2041 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt 2101 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg 2161 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg 2221 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat 2281 cagataaaat atttctagca tgagcggata catatttgaa tgtatttaga aaaataaaca 2341 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gctagggccg gcctacggcc 2401 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga 2461 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga 2521 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg 2581 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagg 2641 acccttgtcc aattaattat tagaaaaatt catccagcat cagatgaaat tgcagtttgt 2701 tcatatccgg attatcaatg ccatatttct gaaacagacg tttttgcagg ctcgggctaa 2761 attcgcccag gcagttccac agaatggcca gatcctgata acgatccgca atgcccacac 2821 ggcccacatc aatgcagcca atcagtttgc cttcatcgaa aatcaggtta tccaggctaa 2881 aatcgccgtg ggtcaccacg ctatccgggc taaacggcag cagtttatgc atttctttcc 2941 acacctgttc caccggccag ccgttacgtt catcatcaaa atcgctcgca tccaccaggc 3001 cgttgttcat acggctctgc gcctgggcca gacgaaacac acgatcgctg ttaaacgggc 3061 agttgcacac cggaatgcta tgcagacgac gcagaaacac ggccagcgca tccacaatgt 3121 tttcgccgct atccggatat tcttccagca cctgaaacgc ggttttgccc ggaatcgcgg 3181 tggtcagcag ccacgcatca tccggggtgc gaataaaatg tttaatggtc ggcagcggca 3241 taaattcggt cagccagttc agacgcacca tttcatcggt cacatcgttc gccacgctgc 3301 ctttgccatg tttcagaaac agttccggcg catccggttt gccatacaga cgataaatgg 3361 tcgcgccgct ctgacccacg ttatcacgcg cccatttata gccatacaga tccgcatcca 3421 tgttgctgtt cagacgcgga cggctacagc tcgtttcacg ctgaatatgg ctcataacac 3481 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat 3541 cttgtgcaat gtaacatcag agattttgag acacaaattt //