LOCUS BBa_K1150018 6856 bp DNA circular UNA 24-OCT-2013 DEFINITION uniCAS, Freiburg 2013. ACCESSION urn.local...1382991826894.24 VERSION urn.local...1382991826894.24 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..344 /note="Geneious type: Promoter" /label="SV40" source <347..359 /label="Präfix" misc_feature 351..4556 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 354..359 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 360..386 /note="Geneious type: Short Element" /label="HA" source 387 /label="Suffix" source <388..392 /label="Präfix" misc_feature 393..413 /note="Geneious type: Short Element" /label="NLS 1" source 414 /label="Suffix" source <415..419 /label="Präfix" misc_feature 2934..2936 /note="Geneious type: Mutation" /label="H840A" misc_feature 4527..4547 /note="Geneious type: Short Element" /label="NLS 2" terminator 4565..4786 /note="Geneious type: Terminator" /label="BgH" misc_feature 4788..4807 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 4807 /label="Suffix" misc_feature 5047..5661 /note="Geneious type: Backbone" /label="Replicon" misc_feature 5946..6605 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 6835..6855 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 6835 /label="Präfix" ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag atggccggct 361 acccatacga tgttccagat tacgctaccg gccctgcaag aaaaagattg aataccggcg 421 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca 481 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca 541 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca 601 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc 661 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg 721 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca 781 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac 841 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga 901 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg 961 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca 1021 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc 1081 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga 1141 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg 1201 ccaaacttca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga 1261 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc 1321 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga 1381 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc 1441 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggat 1501 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa 1561 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc 1621 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca 1681 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga 1741 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat 1801 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg 1861 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc 1921 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata 1981 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg 2041 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga 2101 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg 2161 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca 2221 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga 2281 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc 2341 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc 2401 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt 2461 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc 2521 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg 2581 agcacattgc caatctggca ggcagccccg ccattaagaa gggcatcctg caaacagtga 2641 aggtggtgga cgagctcgtg aaagtgatgg gacggcacaa gcccgagaac atcgtgatcg 2701 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga 2761 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg 2821 aaaacaccca gcttcagaac gagaagctgt acctgtacta cctgcaaaat gggcgggata 2881 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg 2941 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca 3001 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact 3061 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca 3121 aggccgagag aggcggcctg agcgaactgg ataaggccgg wttcatcaag agacagctgg 3181 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta 3241 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc 3301 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc 3361 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc 3421 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga 3481 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca 3541 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc 3601 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca 3661 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga 3721 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca 3781 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt 3841 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag 3901 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc 3961 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact 4021 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgcgggc gaactgcaaa 4081 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact 4141 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc 4201 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc 4261 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca 4321 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg 4381 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg 4441 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc 4501 tgtctcagct gggaggcgac accggccctg caagaaaaag attgaatacc ggttaatact 4561 agaggtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 4621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 4681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 4741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgtact agtagcggcc 4801 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga 4861 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc 4921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5041 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc 5101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5461 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 5521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 5581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5701 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5761 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct 5821 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat 5881 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata 5941 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 6001 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 6061 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 6121 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 6181 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 6241 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 6301 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 6361 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga 6421 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 6481 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 6541 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 6601 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt 6661 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc 6721 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 6781 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc 6841 gcggccgctt ctagag //