LOCUS BBa_K1150017 7092 bp DNA circular UNA 24-OCT-2013 DEFINITION uniCAS, Freiburg 2013. ACCESSION urn.local...1382991826864.23 VERSION urn.local...1382991826864.23 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..580 /note="Geneious type: Promoter" /label="CMV" source <583..595 /label="Präfix" misc_feature 587..4792 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 590..595 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 596..622 /note="Geneious type: Short Element" /label="HA" source 623 /label="Suffix" source <624..628 /label="Präfix" misc_feature 629..649 /note="Geneious type: Short Element" /label="NLS 1" source 650 /label="Suffix" source <651..655 /label="Präfix" misc_feature 3170..3172 /note="Geneious type: Mutation" /label="H840A" misc_feature 4763..4783 /note="Geneious type: Short Element" /label="NLS 2" terminator 4801..5022 /note="Geneious type: Terminator" /label="BgH" misc_feature 5024..5043 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5043 /label="Suffix" misc_feature 5283..5897 /note="Geneious type: Backbone" /label="Replicon" misc_feature 6182..6841 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 7071..7091 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 7071 /label="Präfix" ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagatgg ccggctaccc 601 atacgatgtt ccagattacg ctaccggccc tgcaagaaaa agattgaata ccggcgacaa 661 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga 721 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat 781 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg 841 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca 901 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga 961 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt 1021 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt 1081 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa 1141 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa 1201 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc 1261 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga 1321 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc 1381 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa 1441 acttcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg 1501 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag 1561 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa 1621 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct 1681 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggatacat 1741 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat 1801 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg 1861 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct 1921 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat 1981 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc 2041 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga 2101 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc 2161 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga 2221 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga 2281 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca 2341 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt 2401 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga 2461 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct 2521 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt 2581 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag 2641 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct 2701 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac 2761 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca 2821 cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa cagtgaaggt 2881 ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg tgatcgaaat 2941 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg 3001 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa 3061 cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc gggatatgta 3121 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc 3181 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa 3241 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg 3301 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc 3361 cgagagaggc ggcctgagcg aactggataa ggccggwttc atcaagagac agctggtgga 3421 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta 3481 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt 3541 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca 3601 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa 3661 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc 3721 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat 3781 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat 3841 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt 3901 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg 3961 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa 4021 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt 4081 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct 4141 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga 4201 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct 4261 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac tgcaaaaggg 4321 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga 4381 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa 4441 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc 4501 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag 4561 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc 4621 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct 4681 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc 4741 tcagctggga ggcgacaccg gccctgcaag aaaaagattg aataccggtt aatactagag 4801 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 4861 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 4921 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 4981 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgtactagta gcggccgctg 5041 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag 5101 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 5281 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg 5341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5461 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 5521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5701 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 5761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag 6061 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag 6121 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa 6181 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga 6241 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 6301 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg 6361 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat 6421 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg 6481 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg 6541 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct 6601 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt 6661 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 6721 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 6781 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca 6841 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc 6901 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct 6961 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 7021 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg 7081 ccgcttctag ag //