LOCUS pSB4A5_std__J231 4464 bp ds-DNA circular 17-JUL-2013 DEFINITION Low copy BioBrick standard vector COMMENT Features removed: stop codons, COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature complement(1398..3434) /label=BBa_I50042 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 146..345 /label=Plac promoter - BBa_R0010 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1330..1349) /label=Verification reverse VR /ApEinfo_fwdcolor=#CC33CC /ApEinfo_revcolor=#CC33CC /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 146..345 /label=BBa_R0010 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 234..271 /label=CAP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 282..287 /label=-35 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1236..1248) /label=BBa_B0044 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 306..311 /label=-10 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc 1236..1240 /gene="Nt.BstNB I recognition site" /label=Nt.BstNB I recognition site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 311..345 /label=LacI /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 354..365 /label=BBa_B0034 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 358..361 /label=Conserved: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1249..1260) /label=BBa_B0042 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 372..1052 /label=BBa_E1010 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1261..1329) /label=BBa_B0054 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1053..1077 /label=barcode: /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(1350..1390) /label=BBa_B0062 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1174..1214 /label=BBa_B0012 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 1181..1200 /label=T7 TE /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 stem_loop 1443..1472 /label=Terminator /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 2243..2262 /label=promoter region /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 RBS 2276..2281 /label=RBS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(4393..4464) /label=BBa_B0053 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(21..98) /label=BBa_B0055 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc complement(48..86) /gene="identical to bacteriophage T3" /label=identical to bacteriophage T3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(99..110) /label=BBa_B0042(1) /ApEinfo_label=BBa_B0042 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(111..123) /label=BBa_B0043 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc complement(119..123) /gene="Nt.BstNB I recognition site" /label=Nt.BstNB I recognition site(1) /ApEinfo_label=Nt.BstNB I recognition site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 124..145 /label=BioBrick Prefix /ApEinfo_fwdcolor=#3366FF /ApEinfo_revcolor=#3366FF /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 1215..1235 /label=BioBrick Suffix /ApEinfo_fwdcolor=#3366FF /ApEinfo_revcolor=#3366FF /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 1..20 /label=Verification forward VF2 /ApEinfo_fwdcolor=#CC33CC /ApEinfo_revcolor=#CC33CC /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 124..129 /label=EcoRI /ApEinfo_fwdcolor=#ff3366 /ApEinfo_revcolor=#ff3366 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 139..144 /label=XbaI /ApEinfo_fwdcolor=#ff3366 /ApEinfo_revcolor=#ff3366 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1216..1221 /label=SpeI /ApEinfo_fwdcolor=#ff3366 /ApEinfo_revcolor=#ff3366 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1230..1235 /label=PstI /ApEinfo_fwdcolor=#ff3366 /ApEinfo_revcolor=#ff3366 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 316..338 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2283..3233 /label=RepA /ApEinfo_fwdcolor=#FF6633 /ApEinfo_revcolor=#FF6633 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3522..4385 /label=AmpR2 /ApEinfo_fwdcolor=gold2 /ApEinfo_revcolor=gold2 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1086..1165 /label=BBa_B0010 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 372..1046 /label=mRFP2 /ApEinfo_fwdcolor=red /ApEinfo_revcolor=red /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 1534..1901 /label=Par region /ApEinfo_fwdcolor=#FF6633 /ApEinfo_revcolor=#FF6633 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc 61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga 121 ctcgaattcg cggccgcttc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg 181 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 241 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 301 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg 361 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc 421 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc 481 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg 541 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg 601 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta 661 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg 721 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc 781 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc 841 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag 901 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca 961 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg 1021 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac 1081 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1141 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1201 tttctgcgtt tatatactag tagcggccgc tgcaggagtc actaagggtt agttagttag 1261 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt 1321 atcattgggg ctcactcaaa ggcggtaatc agataaaaaa aatccttagc tttcgctaag 1381 gatgatttct gctagagctg tcagaccaag tttacgagct cgcttggact cctgttgata 1441 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg 1501 gcgtttttta ttggtgagaa tccaagcact agggacagta agacgggtaa gcctgttgat 1561 gataccgctg ccttactggg tgcattagcc agtctgaatg acctgtcacg ggataatccg 1621 aagtggtcag actggaaaat cagagggcag gaactgctga acagcaaaaa gtcagatagc 1681 accacatagc agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat 1741 tatgggtagt ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt 1801 cactgccaga gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg 1861 cctgatggtc ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac 1921 ttaagccttt agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc 1981 ttttgtaata ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc 2041 tttttatctt tttttattct ttctttattc tataaattat aaccacttga atataaacaa 2101 aaaaaacaca caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc 2161 cagcaaaggt ctagcagaat ttacagatac ccacaactca aaggaaaagg acatgtaatt 2221 atcattgact agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat 2281 gtatgtctga attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa 2341 cggagcatga aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa 2401 accctacaag gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga 2461 acatcagtag ggaaaatgct tatggtgtat tagctaaagc aaccagagag ctgatgacga 2521 gaactgtgga aatcaggaat cctttggtta aaggctttga gattttccag tggacaaact 2581 atgccaagtt ctcaagcgaa aaattagaat tagtttttag tgaagagata ttgccttatc 2641 ttttccagtt aaaaaaattc ataaaatata atctggaaca tgttaagtct tttgaaaaca 2701 aatactctat gaggatttat gagtggttat taaaagaact aacacaaaag aaaactcaca 2761 aggcaaatat agagattagc cttgatgaat ttaagttcat gttaatgctt gaaaataact 2821 accatgagtt taaaaggctt aaccaatggg ttttgaaacc aataagtaaa gatttaaaca 2881 cttacagcaa tatgaaattg gtggttgata agcgaggccg cccgactgat acgttgattt 2941 tccaagttga actagataga caaatggatc tcgtaaccga acttgagaac aaccagataa 3001 aaatgaatgg tgacaaaata ccaacaacca ttacatcaga ttcctaccta cataacggac 3061 taagaaaaac actacacgat gctttaactg caaaaattca gctcaccagt tttgaggcaa 3121 aatttttgag tgacatgcaa agtaagtatg atctcaatgg ttcgttctca tggctcacgc 3181 aaaaacaacg aaccacacta gagaacatac tggctaaata cggaaggatc tgaggttctt 3241 atggctcttg tatctatcag tgaagcatca agactaacaa acaaaagtag aacaactgtt 3301 caccgttaca tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag 3361 atagatacat cagagctttt acgagttttt ggtgcattca aagctgttca ccatgaacag 3421 atcgacaatg taactactag agctaaatac attcaaatat ctatccgctc atgagacaat 3481 aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagtatt caacatttcc 3541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 3601 cgctggtgaa agtaaaagat gccgaagatc agttgggtgc acgtgtgggt tacatcgaac 3661 tggacctcaa cagcggtaag attcttgaga gttttcgccc cgaagaacgt ttcccaatga 3721 tgagcacttt taaagttctg ctctgtggcg cggtattatc ccgtattgac gccgggcaag 3781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 3841 cagaaaagca tcttacggac ggcatgacag tacgcgaatt atgcagcgct gccataacca 3901 tgagtgataa cacggcggcc aacttacttc tgacaacgat cggaggaccg aaggagctta 3961 ccgctttttt gcacaacatg ggtgatcatg taactcgcct tgatcgttgg gaaccggagc 4021 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagct atggcaacaa 4081 cgttgcgcaa actcttaact ggcgaacttc ttactctcgc ttcccggcaa caattaatag 4141 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 4201 ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatt attgcagccc 4261 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agccaggcaa 4321 ctatggacga acgtaatcgc cagatcgctg agataggtgc ctccctgatt aagcattggt 4381 aataatacta gctccggcaa aaaaacgggc aaggtgtcac caccctgccc tttttcttta 4441 aaaccgaaaa gattacttcg cgtt //