LOCUS BBa_K1150023 7717 bp DNA circular UNA 23-OCT-2013 DEFINITION uniCAS Histonemodifier, Freiburg 2013. ACCESSION urn.local...1382991827043.29 VERSION urn.local...1382991827043.29 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..344 /note="Geneious type: Promoter" /label="SV40" source <347..359 /label="Präfix" misc_feature 351..4520 /note="Geneious type: dCas9 [RFC 25]" /label="dCas9 [RFC 25]" misc_feature 354..359 /note="Geneious type: Prefix/Suffix" /label="Prefix" misc_feature 360..386 /note="Geneious type: Short Element" /label="HA" source 387 /label="Suffix" source <388..392 /label="Präfix" misc_feature 393..413 /note="Geneious type: Short Element" /label="NLS 1" source 414 /label="Suffix" source <415..419 /label="Präfix" misc_feature 2934..2936 /note="Geneious type: Mutation" /label="H840A" source 4521 /label="Suffix" misc_feature 4522..4540 /note="Geneious type: Short Element" /label="Linker" misc_feature 4542..5417 /note="Geneious type: Effector" /label="G9a [RFC 25]" source <5383..5387 /label="Präfix" misc_feature 5387^5388 /note="Geneious type: origin" /label="origin" misc_feature 5388..5408 /note="Geneious type: Short Element" /label="NLS 2" misc_feature 5409..5414 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5409..>5419 /label="Suffix" terminator 5426..5647 /note="Geneious type: Terminator" /label="BgH" misc_feature 5649..5668 /note="Geneious type: Prefix/Suffix" /label="Suffix" source 5668 /label="Suffix" misc_feature 5908..6522 /note="Geneious type: Backbone" /label="Replicon" misc_feature 6807..7466 /note="Geneious type: Backbone" /label="Chloramphenicol resistance" misc_feature 7696..7716 /note="Geneious type: Prefix/Suffix" /label="Prefix" source 7696 /label="Präfix" ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag atggccggct 361 acccatacga tgttccagat tacgctaccg gccctgcaag aaaaagattg aataccggcg 421 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca 481 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca 541 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca 601 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc 661 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg 721 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca 781 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac 841 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga 901 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg 961 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca 1021 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc 1081 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga 1141 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg 1201 ccaaacttca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga 1261 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc 1321 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga 1381 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc 1441 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggat 1501 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa 1561 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc 1621 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca 1681 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga 1741 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat 1801 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg 1861 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc 1921 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata 1981 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg 2041 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga 2101 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg 2161 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca 2221 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga 2281 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc 2341 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc 2401 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt 2461 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc 2521 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg 2581 agcacattgc caatctggca ggcagccccg ccattaagaa gggcatcctg caaacagtga 2641 aggtggtgga cgagctcgtg aaagtgatgg gacggcacaa gcccgagaac atcgtgatcg 2701 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga 2761 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg 2821 aaaacaccca gcttcagaac gagaagctgt acctgtacta cctgcaaaat gggcgggata 2881 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg 2941 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca 3001 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact 3061 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca 3121 aggccgagag aggcggcctg agcgaactgg ataaggccgg wttcatcaag agacagctgg 3181 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta 3241 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc 3301 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc 3361 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc 3421 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga 3481 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca 3541 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc 3601 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca 3661 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga 3721 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca 3781 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt 3841 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag 3901 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc 3961 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact 4021 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgcgggc gaactgcaaa 4081 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact 4141 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc 4201 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc 4261 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca 4321 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg 4381 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg 4441 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc 4501 tgtctcagct gggaggcgac accggcgcag gctccaccgg cgagaatgta cccatcccct 4561 gtgtcaatgg tgtggatggg gagccgtgcc cggaggacta caagtacatc tctgagaact 4621 gcgagacatc gaccatgaac atcgaccgca acatcaccca tctccagcac tgcacgtgtg 4681 tggatgactg ctccagctcc aattgcctat gtggtcagct cagtatccga tgctggtatg 4741 acaaggacgg gcggctgctc caggagttta acaagatcga gccccccctg atctttgagt 4801 gtaaccaggc atgctcctgc tggagaagct gcaagaaccg cgtggtgcag agcggcatca 4861 aggtacggct ccagctctac cggactgcca agatgggctg gggggtccga gccttgcaga 4921 ccatccccca gggcacgttc atctgcgagt atgtaggaga gctgatctct gatgccgagg 4981 ctgatgtgag agaggatgat tcttacctct tcgatttaga taacaaggat ggcgaggttt 5041 actgcattga tgcccgttac tatggcaaca tcagccgatt cattaaccac ctgtgtgacc 5101 ccaacatcat ccctgtccgg gttttcatgc tgcaccaaga tctacggttc ccacgcattg 5161 ccttcttcag ctccagggac atccggactg gggaggagct gggctttgac tacggtgacc 5221 gattctggga catcaagagc aagtatttca cctgccagtg tggctctgag aagtgcaagc 5281 attcagcgga ggccatcgcc ctggagcaga gccgcctggc ccggctggac ccccacccgg 5341 agctgctccc tgacctcagc tccctgcccc ccatcaacac caccggccct gcaagaaaaa 5401 gattgaatac cggttaatac tagaggtgcc ttctagttgc cagccatctg ttgtttgccc 5461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 5521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 5581 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 5641 ctctatgtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct 5701 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg 5761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 5821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 5881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc 5941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 6361 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 6421 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 6481 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 6541 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 6601 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 6661 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc 6721 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca 6781 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt 6841 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 6901 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 6961 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 7021 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 7081 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 7141 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 7201 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga 7261 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 7321 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 7381 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 7441 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac 7501 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg 7561 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 7621 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc 7681 taaggatgat ttctggaatt cgcggccgct tctagag //