LOCUS       pSB3T5_std_             3255 bp ds-DNA     circular     18-JUL-2013
DEFINITION  Low to medium copy BioBrick standard vector
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(170..182)
                     /label=BBa_B0044
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(183..194)
                     /label=BBa_B0042
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(195..263)
                     /label=BBa_B0054
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(264..283)
                     /label=BBa_G00102
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(284..324)
                     /label=BBa_B0062
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(325..331)
                     /label=BBa_G0003
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(332..1885)
                     /label=BBa_I50032
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            730..1554
                     /gene="p15A origin of replication"
                     /label=p15A origin of replication
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1886..1893)
                     /label=BBa_G0002
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1894..3176)
                     /label=BBa_P1005
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1983..3176
                     /gene="tetR"
                     /label=tetR
                     /ApEinfo_fwdcolor=#eec900
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3177..3183)
                     /label=BBa_G0004
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3184..3255)
                     /label=BBa_B0053
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    149..169
                     /label=BioBrick Suffix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(264..283)
                     /label=Verification reverse VR
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    150..155
                     /label=SpeI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    164..169
                     /label=PstI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(730..1554)
                     /label=p15A Origin (Rev)
                     /ApEinfo_fwdcolor=#FF6633
                     /ApEinfo_revcolor=#FF6633
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1..20)
                     /label=BBa_G00100
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(21..98)
                     /label=BBa_B0055
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(99..110)
                     /label=BBa_B0042(1)
                     /ApEinfo_label=BBa_B0042
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(111..123)
                     /label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    124..145
                     /label=BioBrick Prefix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..20
                     /label=Verification forward VF2
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    124..129
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    139..144
                     /label=XbaI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagnnnta ctagtagcgg ccgctgcagg agtcactaag
      181 ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt ttgactaaaa
      241 cttcccttgg ggttatcatt ggggctcact caaaggcggt aatcagataa aaaaaatcct
      301 tagctttcgc taaggatgat ttctgctaga gatggaatag actggatgga ggcggataaa
      361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      421 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      721 tcagacccct taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc
      781 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac
      841 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt
      901 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc
      961 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     1021 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     1081 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     1141 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca ggggaaacgc
     1201 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     1261 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     1321 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     1381 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     1441 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     1501 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg
     1561 aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc
     1621 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt
     1681 gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg
     1741 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct
     1801 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1861 cataaacagt aatacaaggg gtgtttacta gaggagattc tcatgtttga cagcttatca
     1921 tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt
     1981 gtatgaaatc taacaatgcg ctcatcgtca ttctcggcac cgtcaccctg gacgctgtag
     2041 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca
     2101 gtattgccag tcactatggc gtgctgcttg cgctctatgc gttgatgcaa tttctttgcg
     2161 cacccgttct cggagccctg tccgaccgct ttggccgccg tccagtcctg ctcgcttcgc
     2221 tccttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggattctct
     2281 acgccggacg catcgtggcg ggcatcacgg gtgccacagg tgcggttgct ggtgcctata
     2341 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     2401 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggtgcc atctccttgc
     2461 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctcctcctg ggctgcttcc
     2521 ttatgcagga atcgcataag ggagagcgcc gtccgatgcc cttgcgtgcc ttcaatccag
     2581 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgttttct
     2641 ttatcatgca actcgtagga caggttccgg cagcgctctg ggtcattttc ggcgaggacc
     2701 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
     2761 ccctcgctca agccttcgtc acgggccccg ccaccaaacg tttcggcgag aagcaggcca
     2821 ttatcgcggg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgcg
     2881 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggt atgcccgcgt
     2941 tgcaggccat gctgtcccgc caagtagatg acgaccatca gggacagctt caagggtcgc
     3001 tcgcggctct taccagcctc acttcgatca ttggaccgct gatcgtcacg gcgatttatg
     3061 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggtgccgcc ctttaccttg
     3121 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc taataatact
     3181 agctccggca aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     3241 agattacttc gcgtt    
//
