LOCUS       pSB1A3_std_             2160 bp ds-DNA     circular     24-SEP-2013
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     http://partsregistry.org/Part:pSB1A3
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     stem_loop       173..207
                     /label=terminator stem loop
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     prim_transcript 165..236
                     /label=E.coli his operon terminator
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(404..1116)
                     /label=rep(pMB1)
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    119..140
                     /label=BioBrick Prefix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    144..164
                     /label=BioBrick Suffix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..20
                     /label=Verification forward VF2 primer binding site
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(300..319)
                     /label=Verification reverse VR primer binding site
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    119..124
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    134..139
                     /label=XbaI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    145..150
                     /label=SpeI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    159..164
                     /label=PstI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1181..1840)
                     /label=AmpR
                     /ApEinfo_fwdcolor=gold2
                     /ApEinfo_revcolor=gold2
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(401..1083)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=#FF6633
                     /ApEinfo_revcolor=#FF6633
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TGCCACCTGA CGTCTAAGAA accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgGA
      121 ATTCGCGGCC GCTTCTAGAG nnnTACTAGT AGCGGCCGCT GCAGtccggc aaaaaaacgg
      181 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      241 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcaG
      301 CTCACTCAAA GGCGGTAATa cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      841 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1141 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1201 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1261 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1321 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1381 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1441 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1501 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1561 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1621 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1681 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1741 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1801 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1861 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1921 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1981 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2041 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2101 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaaG
//
