LOCUS       pSB1C3_std__sfGF        2857 bp ds-DNA     circular     15-JUL-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pSB1C3 (std) from 1 to 2079
COMMENT     from: http://partsregistry.org/Part:BBa_K777000
COMMENT     plus: http://parts.igem.org/wiki/index.php/Part:BBa_K515105
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(2087..2746)
                     /label=Chloramphenicol resistance
                     /ApEinfo_fwdcolor=#e4c903
                     /ApEinfo_revcolor=#e4c903
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(141..175)
                     /label=BBa_J23100
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             184..207
                     /label=rbs
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    119..140
                     /label=BioBrick Prefix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    928..948
                     /label=BioBrick Suffix
                     /ApEinfo_fwdcolor=#3366FF
                     /ApEinfo_revcolor=#3366FF
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..20
                     /label=Verification forward VF2 primer binding site
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(1082..1101)
                     /label=Verification reverse VR primer binding site
                     /ApEinfo_fwdcolor=#CC33CC
                     /ApEinfo_revcolor=#CC33CC
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    119..124
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    134..139
                     /label=XbaI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    929..934
                     /label=SpeI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    943..948
                     /label=PstI
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=#ff3366
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             208..927
                     /label=BBa_K515005 - Superfolder GFP
                     /ApEinfo_fwdcolor=green
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             184..207
                     /label=BBa_K515005 - RBS
                     /ApEinfo_fwdcolor=green
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(2090..2161,2163..2527,2529..2746))
                     /label=CmR2
                     /ApEinfo_fwdcolor=gold2
                     /ApEinfo_revcolor=gold2
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(1188..1794)
                     /label=rep (pMB1) ORI
                     /ApEinfo_fwdcolor=#FF6633
                     /ApEinfo_revcolor=#FF6633
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TGCCACCTGA CGTCTAAGAA accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgGA
      121 ATTCGCGGCC GCTTCTAGAG ttgacggcta gctcagtcct aggtacagtg ctagctacta
      181 gagaaatcaa attaaggagg taagataatg cgtaaaggcg aagaactgtt cacgggcgta
      241 gttccgattc tggtcgagct ggacggcgat gtgaacggtc ataagtttag cgttcgcggt
      301 gaaggtgagg gcgacgcgac caacggcaaa ctgaccctga agttcatctg caccaccggt
      361 aaactgccgg tgccttggcc gaccttggtg acgacgttga cgtatggcgt gcagtgtttt
      421 gcgcgttatc cggaccacat gaaacaacac gatttcttca aatctgcgat gccggagggt
      481 tacgtccagg agcgtaccat ttccttcaag gatgatggct actacaaaac tcgcgcagag
      541 gttaagtttg aaggtgacac gctggtcaat cgtatcgaat tgaagggtat cgactttaaa
      601 gaggatggta acattctggg ccataaactg gagtataact tcaacagcca taatgtttac
      661 attacggcag acaagcaaaa gaacggcatc aaggccaatt tcaagattcg ccacaatgtt
      721 gaggacggta gcgtccaact ggccgaccat taccagcaga acaccccaat tggtgacggt
      781 ccggttttgc tgccggataa tcactatctg agcacccaaa gcgtgctgag caaagatccg
      841 aacgaaaaac gtgatcacat ggtcctgctg gaatttgtga ccgctgcggg catcacccac
      901 ggtatggacg agctgtataa gtaatgaTAC TAGTAGCGGC CGCTGCAGtc cggcaaaaaa
      961 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     1021 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 aGCTCACTCA AAGGCGGTAA Tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     1981 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     2041 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaaTTAc gccccgccct
     2101 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2161 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2221 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2281 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2341 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2401 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2461 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2521 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2581 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2641 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2701 attgggatat atcaacggtg gtatatccag tgattttttt ctcCATttta gcttccttag
     2761 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2821 gaaagttgga acctcttacg tgcccgatca actcgag  
//
