LOCUS                  pEO100c        5266 bp ds-DNA   circular     27-APR-2011
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5245..5266
                     /label=bb prefix
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    1930..1950
                     /label=bb suffix
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    1..108
                     /label=R0082
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
     misc_feature    192..977
                     /label=ompC' fragment from RU1012
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=#ff0080
     CDS             complement(4150..4809)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     rep_origin      2309..3260
                     /label=pSC101* (-)
                     /ApEinfo_fwdcolor=#ff7e07
                     /ApEinfo_revcolor=#ff7e07
     promoter        3281..3299
                     /label=pSC101* promoter (-)
                     /ApEinfo_fwdcolor=#ff5007
                     /ApEinfo_revcolor=#ff5007
     primer_bind     2084..2103
                     /label=G00101 binding site
                     /ApEinfo_fwdcolor=#ff6612
                     /ApEinfo_revcolor=#ff6612
     primer_bind     5127..5146
                     /label=G00100 binding site
                     /ApEinfo_fwdcolor=#ff6612
                     /ApEinfo_revcolor=#ff6612
     terminator      4016..4121
                     /label=T0
                     /ApEinfo_fwdcolor=#ff0517
                     /ApEinfo_revcolor=#ff0517
     misc_feature    1..1157
                     /label=PompC1157
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1039..1058
                     /label=Sequencing primer
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1190..1909
                     /label=superfolder GFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
     misc_feature    1158..1189
                     /label=sRBS (100000)
                     /ApEinfo_fwdcolor=#ec0076
                     /ApEinfo_revcolor=green
     misc_feature    313..313
                     /label=mutated to G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    974..974
                     /label=C deleted here (110427)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
ORIGIN
        1 TCCCTTGCAT TTACATTTTG AAACATCTAT AGCGATAAAT GAAACATCTT AAAAGTTTTA
       61 GTATCATATT CGTGTTGGAT TATTCTGCAT TTTTGGGGAG AATGGACTTG CCGACTGATT
      121 AATGAGGGTT AATCAGTATG CAGTGGCATA AAAAAGCAAA TAAAGGCATA TAACAGAGGG
      181 TTAATAACAT GAAAGTTAAA GTACTGTCCC TCCTGGTCCC AGCTCTGCTG GTAGCAGGCG
      241 CAGCAAACGC TGCTGAAGTT TACAACAAAG ACGGCAACAA ATTAGATCTG TACGGTAAAG
      301 TAGACGGCCT GCACTATTTC TCTGACAACA AAGATGTAGA TGGCGACCAG ACCTACATGC
      361 GTCTTGGCTT CAAAGGTGAA ACTCAGGTTA CTGACCAGCT GACCGGTTAC GGCCAGTGGG
      421 AATATCAGAT CCAGGGCAAC AGCGCTGAAA ACGAAAACAA CTCCTGGACC CGTGTGGCAT
      481 TCGCAGGTCT GAAATTCCAG GATGTGGGTT CTTTCGACTA CGGTCGTAAC TACGGCGTTG
      541 TTTATGACGT AACTTCCTGG ACCGACGTAC TGCCAGAATT CGGTGGTGAC ACCTACGGTT
      601 CTGACAACTT CATGCAGCAG CGTGGTAACG GCTTCGCGAC CTACCGTAAC ACTGACTTCT
      661 TCGGTCTGGT TGACGGCCTG AACTTTGCTG TTCAGTACCA GGGTAAAAAC GGCAACCCAT
      721 CTGGTGAAGG CTTTACTAGT GGCGTAACTA ACAACGGTCG TGACGCACTG CGTCAAAACG
      781 GCGACGGCGT CGGCGGTTCT ATCACTTATG ATTACGAAGG TTTCGGTATC GGTGGTGCGA
      841 TCTCCAGCTC CAAACGTACT GATGCTCAGA ACACCGCTGC TTACATCGGT AACGGCGACC
      901 GTGCTGAAAC CTACACTGGT GGTCTGAAAT ACGACGCTAA CAACATCTAC CTGGCTGCTC
      961 AGTACACCCA GACCTACTGA AGCGGCGCAC GAAAAACGCG AAAGCTAAAG TTTTCGCATT
     1021 TATCGTGAAA CGCTTTCGCG TTTTTCGTGC GCCGCTTCAC CGGATAAAGA GCAGCTACTG
     1081 GTGGTTAACC TTTCCGGTCG CGGCGATAAA GACATCTTCA CCGTTCACGA TATTTTGAAA
     1141 GCACGAGGGG AAATCTGTCA TATATAAATA AAATAAGGTA GGTCAATATa tgcgtaaagg
     1201 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     1261 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     1321 gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct
     1381 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     1441 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     1501 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     1561 gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa
     1621 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     1681 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     1741 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     1801 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     1861 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgag cccgtatttc
     1921 gcgtaaggat actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc
     1981 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
     2041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2101 aatctcgagg ttacattgtc gatctgttca tggtgaacag ctttaaatgc accaaaaact
     2161 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt
     2221 tttccctttg atatctaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     2281 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     2341 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt
     2401 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     2461 agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag gaatctgatg taatggttgt
     2521 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     2581 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     2641 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     2701 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2761 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2821 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2881 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2941 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     3001 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     3061 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     3121 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     3181 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     3241 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     3301 attgagatgg gctagtcaat gataattaca tgtccttttc ctttgagttg tgggtatctg
     3361 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     3421 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     3481 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     3541 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     3601 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     3661 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3721 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     3781 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     3841 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
     3901 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
     3961 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt
     4021 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     4081 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt aaacttggtc
     4141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4261 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5101 aggggttccg cgcacatttc ccatggtgcc acctgacgtc taagaaacca ttattatcat
     5161 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     5221 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagag 
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