LOCUS       PhPFDa.xdna    2863 bp      DNA     circular   13/08/10
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             complement(1904..2764)
                     /label=AmpR
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     322..341
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(1127..1755)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(2806..2834)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     396..415
                     /label=Biobrick prefix 
                     /SerialCloner_Color=&h66FFCC
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     876..895
                     /label=Biobrick suffix
                     /SerialCloner_Color=&h66FFCC
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             416..871
                     /label=PhPFDa
                     /SerialCloner_Color=&h0008FD
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 aACTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC 
       61 TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC 
      121 GAGATAGGGT TGAGTGGCCG CTACAGGGCG CTCCCATTCG CCATTCAGGC TGCGCAACTG 
      181 TTGGGAAGGG CGTTTCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT 
      241 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA 
      301 CGACGGCCAG TGAGCGCGAC GTAATACGAC TCACTATAGG GCGAATTGGC GGAAGGCCGT 
      361 CAAGGCCACG TGTCTTGTCC AGGTACCGTT TCTTCGAATT CGCGGCCGCT TCTAGATGAT 
      421 CCGTATGGCT CAGAACAACA AAGAACTGGA AAAACTGGCT TACGAATACC AGGTTCTGCA 
      481 AGCTCAGGCT CAGATCCTGG CTCAGAACCT GGAACTGCTG AACCTGGCTA AAGCTGAAGT 
      541 TCAGACCGTT CGTGAAACCC TGGAAAACCT GAAAAAAATC GAAGAAGAAA AACCGGAAAT 
      601 CCTGGTTCCG ATCGGTGCTG GTTCTTTCCT GAAAGGTGTT ATCGTTGACA AAAACAACGC 
      661 TATCGTTTCT GTTGGTTCTG GTTACGCTGT TGAACGTTCT ATCGACGAAG CTATCTCTTT 
      721 CCTGGAAAAA CGTCTGAAAG AATACGACGA AGCTATCAAA AAAACCCAGG GTGCTCTGGC 
      781 TGAACTGGAA AAACGTATCG GTGAAGTTGC TCGTAAAGCT CAGGAAGTTC AGCAGAAACA 
      841 GTCTATGACC TCTTTCAAAG TTAAAAAATA ATAATACTAG TAGCGGCCGC TGCAGGAAGA 
      901 AACGAGCTCT GGAGCACAAG ACTGGCCTCA TGGGCCTTCC GCTCACTGCC CGCTTTCCAG 
      961 TCGGGAAACC TGTCGTGCCA GCTGCATTAA CATGGTCATA GCTGTTTCCT TGCGTATTGG 
     1021 GCGCTCTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGGT AAAGCCTGGG 
     1081 GTGCCTAATG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     1141 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     1201 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     1261 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     1321 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     1381 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     1441 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     1501 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     1561 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     1621 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     1681 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     1741 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     1801 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     1861 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     1921 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     1981 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     2041 CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     2101 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     2161 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     2221 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     2281 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     2341 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     2401 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     2461 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     2521 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     2581 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     2641 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     2701 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     2761 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     2821 GATACATATT TGAATGTATT TAGAAAAATA AAGTGCCACc taa
//