LOCUS       406C.xdna    3778 bp      DNA     circular   13/08/10
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pSB3C5.xdna [2697  nt] : (PstI[54] / EcoRI[275
            5]) to J23109.xdna [54  nt] : (EcoRI[2962] / SpeI[37]) and t
            o 405A.xdna [1015  nt] : (XbaI[2174] / PstI[3193])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     terminator      27..95
                     /label=B0054
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      2502..2573
                     /label=B0053
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      15..26
                     /label=B0042
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      2672..2683
                     /label=B0042
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      116..156
                     /label=B0062
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      2594..2671
                     /label=B0055
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      3717..3758
                     /label=B0012
                     /SerialCloner_Color=&h996633
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     terminator      3628..3758
                     /label=B0015
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     RBS             2761..2774
                     /label=J61107
                     /SerialCloner_Color=&hFA00FA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     RBS             3246..3259
                     /label=J61107
                     /SerialCloner_Color=&hFA00FA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    3759..1
                     /label=Biobrick suffix
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     2574..2593
                     /label=G00100
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(96..115)
                     /label=G00102
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      27..95
                     /label=B0054
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2502..2573
                     /label=B0053
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      15..26
                     /label=B0042
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2672..2683
                     /label=B0042
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      116..156
                     /label=B0062
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      164..1717
                     /label=I50032
                     /SerialCloner_Color=&h0008FD
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2594..2671
                     /label=B0055
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1726..2494
                     /label=P1004
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2502..2573
                     /label=Ecoli his terminator
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2511..2545
                     /label=terminator stem loop
                     /SerialCloner_Color=&hC0ABA4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      130..149
                     /label=terminator
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     repeat_region   1570..1613
                     /label=repeat region
                     /SerialCloner_Color=&hC0ABA4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      15..26
                     /label=STOP
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2672..2683
                     /label=STOP
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     3759..1
                     /label=G00001
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     2574..2593
                     /label=G00100m
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     1383..1388
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     2725..2730
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     2748..2753
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(1383..1388)
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(2725..2730)
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(2748..2753)
                     /label=B0045
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3629..3709
                     /label=B0010
                     /SerialCloner_Color=&h996633
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3717..3758
                     /label=B0012
                     /SerialCloner_Color=&h996633
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3628..3758
                     /label=B0015
                     /SerialCloner_Color=&hD08915
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        2719..2753
                     /label=J23109
                     /SerialCloner_Color=&hC03B3D
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     RBS             2761..2774
                     /label=J61107
                     /SerialCloner_Color=&hFA00FA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     RBS             3246..3259
                     /label=J61107
                     /SerialCloner_Color=&hFA00FA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     2697..2718
                     /label=G00000
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     2697..2716
                     /label=G00000
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2780..3235
                     /label=PhPFDa
                     /SerialCloner_Color=&h4E51AA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3265..3618
                     /label=PhPFDb
                     /SerialCloner_Color=&h4E51AA
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 GGAGTCACTA AGGGTTAGTT AGTTAGATTA GCAGAAAGTC AAAAGCCTCC GACCGGAGGC 
       61 TTTTGACTAA AACTTCCCTT GGGGTTATCA TTGGGGCTCA CTCAAAGGCG GTAATCAGAT 
      121 AAAAAAAATC CTTAGCTTTC GCTAAGGATG ATTTCTGCTA GAGATGGAAT AGACTGGATG 
      181 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT 
      241 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA 
      301 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT 
      361 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA 
      421 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG 
      481 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 
      541 TTCCACTGAG CGTCAGACCC CTTAATAAGA TGATCTTCTT GAGATCGTTT TGGTCTGCGC 
      601 GTAATCTCTT GCTCTGAAAA CGAAAAAACC GCCTTGCAGG GCGGTTTTTC GAAGGTTCTC 
      661 TGAGCTACCA ACTCTTTGAA CCGAGGTAAC TGGCTTGGAG GAGCGCAGTC ACCAAAACTT 
      721 GTCCTTTCAG TTTAGCCTTA ACCGGCGCAT GACTTCAAGA CTAACTCCTC TAAATCAATT 
      781 ACCAGTGGCT GCTGCCAGTG GTGCTTTTGC ATGTCTTTCC GGGTTGGACT CAAGACGATA 
      841 GTTACCGGAT AAGGCGCAGC GGTCGGACTG AACGGGGGGT TCGTGCATAC AGTCCAGCTT 
      901 GGAGCGAACT GCCTACCCGG AACTGAGTGT CAGGCGTGGA ATGAGACAAA CGCGGCCATA 
      961 ACAGCGGAAT GACACCGGTA AACCGAAAGG CAGGAACAGG AGAGCGCACG AGGGAGCCGC 
     1021 CAGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG 
     1081 TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC 
     1141 CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG 
     1201 TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA 
     1261 GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG 
     1321 CCACATGAAG CACTTCACTG ACACCCTCAT CAGTGCCAAC ATAGTAAGCC AGTATACACT 
     1381 CCGCTAGCGC TGAGGTCTGC CTCGTGAAGA AGGTGTTGCT GACTCATACC AGGCCTGAAT 
     1441 CGCCCCATCA TCCAGCCAGA AAGTGAGGGA GCCACGGTTG ATGAGAGCTT TGTTGTAGGT 
     1501 GGACCAGTTG GTGATTTTGA ACTTTTGCTT TGCCACGGAA CGGTCTGCGT TGTCGGGAAG 
     1561 ATGCGTGATC TGATCCTTCA ACTCAGCAAA AGTTCGATTT ATTCAACAAA GCCACGTTGT 
     1621 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA 
     1681 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTTAC TAGAGGTTGA TCGGGCACGT 
     1741 AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT 
     1801 ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACGG GATATACCAC 
     1861 CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA 
     1921 ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA 
     1981 AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAACGCTCA 
     2041 CCCGGAGTTT CGTATGGCCA TGAAAGACGG TGAGCTGGTG ATCTGGGATA GTGTTCACCC 
     2101 TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCG TCCCTCTGGA GTGAATACCA 
     2161 CGACGATTTC CGGCAGTTTC TCCACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA 
     2221 CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTTGTCTCAG CCAATCCCTG 
     2281 GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT 
     2341 TTTCACGATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATCCA 
     2401 GGTTCATCAT GCCGTTTGTG ATGGCTTCCA TGTCGGCCGC ATGCTTAATG AATTACAACA 
     2461 GTACTGTGAT GAGTGGCAGG GCGGGGCGTA ATAATACTAG CTCCGGCAAA AAAACGGGCA 
     2521 AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG ATTACTTCGC GTTTGCCACC 
     2581 TGACGTCTAA GAAAAGGAAT ATTCAGCAAT TTGCCCGTGC CGAAGAAAGG CCCACCCGTG 
     2641 AAGGTGAGCC AGTGAGTTGA TTGCTACGTA ATTAGTTAGT TAGCCCTTAG TGACTCgaat 
     2701 tcgcggccgc atctagagtt tacagctagc tcagtcctag ggactgtgct agctactagA 
     2761 GAAAGAAGAG ACTCActagA TGATCCGTAT GGCTCAGAAC AACAAAGAAC TGGAAAAACT 
     2821 GGCTTACGAA TACCAGGTTC TGCAAGCTCA GGCTCAGATC CTGGCTCAGA ACCTGGAACT 
     2881 GCTGAACCTG GCTAAAGCTG AAGTTCAGAC CGTTCGTGAA ACCCTGGAAA ACCTGAAAAA 
     2941 AATCGAAGAA GAAAAACCGG AAATCCTGGT TCCGATCGGT GCTGGTTCTT TCCTGAAAGG 
     3001 TGTTATCGTT GACAAAAACA ACGCTATCGT TTCTGTTGGT TCTGGTTACG CTGTTGAACG 
     3061 TTCTATCGAC GAAGCTATCT CTTTCCTGGA AAAACGTCTG AAAGAATACG ACGAAGCTAT 
     3121 CAAAAAAACC CAGGGTGCTC TGGCTGAACT GGAAAAACGT ATCGGTGAAG TTGCTCGTAA 
     3181 AGCTCAGGAA GTTCAGCAGA AACAGTCTAT GACCTCTTTC AAAGTTAAAA AATAATAATA 
     3241 ctagAGAAAG AAGAGACTCA ctagATGCAG AACATCCCGC CGCAGGTTCA GGCTATGCTG 
     3301 GGTCAGCTGG ACACCTACCA GCAGCAGCTG CAACTGGTTA TCCAGCAGAA ACAGAAAGTT 
     3361 CAGGCTGACC TGAACGAAGC TAAAAAAGCT CTGGAAGAAA TCGAAACCCT GCCGGACGAC 
     3421 GCTCAGATAT ACAAAACCGT TGGTACCCTG ATCGTTAAAA CCACCAAAGA AAAAGCTGTT 
     3481 CAGGAACTGA AAGAAAAAAT CGAAACCCTG GAAGTTCGTC TGAACGCTCT GAACCGTCAG 
     3541 GAACAGAAAA TCAACGAAAA AGTTAAAGAA CTGACCCAGA AAATCCAGGC TGCTCTGCGT 
     3601 CCGCCGACCG CTGGTTAATA ATActagagc caggcatcaa ataaaacgaa aggctcagtc 
     3661 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 
     3721 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagtagcgg ccgctgca
//