LOCUS FluA+His+middell 3589 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of FluA+His+middellinker+Foki into pMA2_NgoMIV_SpeI ACCESSION FluA+His+middell KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3589) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: FluA+His+middellinker+Foki Fragment ends: NgoMIV and SpeI Fragment size: 1177 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2017..3186 /gene="'ORF-1" /SECDrawAs="Gene" CDS 2017..2034 /gene="His Tag" /SECDrawAs="Gene" CDS 2041..2562 /gene="FluA" /SECDrawAs="Gene" CDS 2566..2592 /gene="'middelLinker38" /SECDrawAs="Gene" CDS 2596..3183 /gene="'FokI" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc 2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc 2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc 2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac 2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag 2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac 2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac 2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag 2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg 2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggcgg tggttctggt 2581 ggtggttctg gtaccggcaa atctgaactg gaggagaaaa aatccgagct gcgccacaaa 2641 ctgaaatatg tgcctcacga gtatatcgaa ctgatcgaga tcgcccgtaa tagtacccaa 2701 gaccgtatcc tggaaatgaa agtgatggag ttcttcatga aagtctatgg ctatcgtggc 2761 aaacatctgg gtggtagccg taaaccagca ggtgccattt ataccgttgg ttccccgatc 2821 gattatggcg ttatcgtggc cacaaaagcg tattctggcg gttataatct gccgattggt 2881 caggctgatg agatggaacg ttatgtggaa gagaatcaga cccgtaacaa acatctgaac 2941 ccgaacgaat ggtggaaagt gtatccgtca agtgtcaccg agttcaaatt tctgttcgtg 3001 agcggccact ttaaaggcaa ctataaagcc cagctgactc gtctgaacca tatcaccaat 3061 agcaatgggg cagtgctgag tgttgaggaa ctgctgatcg gtggagaaat gatcaaagca 3121 ggcaccctga ctctggaaga agttcgccgt aaattcaaca atggcgagat caattttacc 3181 ggttaatact agtagcggcc gctgcagagc tcatggcgcg cctaggcctt gacggccttc 3241 cgccaattcg ccctatagtg agtcgtatta cgtcgcgctc actggccgtc gttttacaac 3301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 3361 tcgccagctg gcgtaatagc gaagaggccc gcaccgaaac gcccttccca acagttgcgc 3421 agcctgaatg gcgaatggga gcgccctgta gcggccactc aaccctatct cggtctattc 3481 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 3541 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttag //