LOCUS His+Dig+middelli 3619 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of His+Dig+middellinker+FokA into pMA2_NgoMIV_SpeI ACCESSION His+Dig+middelli KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3619) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: His+Dig+middellinker+FokA Fragment ends: NgoMIV and SpeI Fragment size: 1207 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2017..3216 /gene="'ORF-1" /SECDrawAs="Gene" CDS 2017..2034 /gene="His Tag" /SECDrawAs="Gene" CDS 2038..2593 /gene="'Dig'" /SECDrawAs="Gene" CDS 2596..2622 /gene="'middelLinker38" /SECDrawAs="Gene" CDS 2626..3213 /gene="'FokA" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc 2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc 2101 cagtaccacg gcaagtggtg gcaggtggcc gcttatcccg accacatcac caagtacggc 2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc ccggtacagc 2221 gtgatccacg gcaaagagta cttcagcgag ggcaccgcct accctgtggg cgacagcaag 2281 atcggcaaga tctaccacag ctacaccatc ggcggcgtga cccaggtggg cgtgagcaac 2341 gtgctgtcca ccgacaacaa gaactacatc atcggctact tttgcagata cgacgaggac 2401 aagaagggcc actgggacgc cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag 2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg 2521 gtgtacagcg acttctccga ggccgcctgc aaagtgaaca acagcaactg gtcccacccc 2581 cagttcgaaa agaccggcgg tggttctggt ggtggttctg gtaccggcaa atctgaactg 2641 gaggagaaaa aatccgagct gcgccacaaa ctgaaatatg tgcctcacga gtatatcgaa 2701 ctgatcgaga tcgcccgtaa tagtacccaa gaccgtatcc tggaaatgaa agtgatggag 2761 ttcttcatga aagtctatgg ctatcgtggc aaacatctgg gtggtagccg taaacctgat 2821 ggtgccattt ataccgttgg ttccccgatc gattatggcg ttatcgttga taccaaagcc 2881 tatagcgggg gttataacct gccaattggt caggctgatg agatgcagcg ttatgtgaaa 2941 gagaaccaga ctcgtaacaa acacatcaac ccgaacgaat ggtggaaagt gtatccgtca 3001 agcgttacag agttcaaatt cctgttcgtg agcggccatt ttaaaggcaa ctataaagca 3061 cagctgaccc gtctgaacca taaaaccaat agcaatggcg ccgttctgtc agtagaagag 3121 ctgctgattg gcggtgaaat gatcaaagcc gggaccctga cactggaaga agttcgccgt 3181 aaattcaaca atggggagat caattttacc ggttaatact agtagcggcc gctgcagagc 3241 tcatggcgcg cctaggcctt gacggccttc cgccaattcg ccctatagtg agtcgtatta 3301 cgtcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 3361 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 3421 gcaccgaaac gcccttccca acagttgcgc agcctgaatg gcgaatggga gcgccctgta 3481 gcggccactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 3541 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 3601 attaacgctt acaatttag //