LOCUS Strep+Dig_pMA 3010 bp DNA circular SYN 20-OCT-2009 DEFINITION Ligation of Dig/pMA (geschnitten) into Strep/pMA (geschnitten) ACCESSION Strep+Dig_pMA KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3010) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: Strep/pMA (geschnitten) Fragment ends: PstI and AgeI Fragment size: 2424 Insert molecule: Dig/pMA (geschnitten) Fragment ends: NgoMIV and PstI Fragment size: 586 FEATURES Location/Qualifiers CDS 512..1372 /gene="AmpR" /SECDrawAs="Gene" CDS 2391..2990 /gene="ORF-1" /SECDrawAs="Gene" CDS 2397..2424 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2427..2982 /gene="'Dig'" /SECDrawAs="Gene" ORIGIN 1 gagctcatgg cgcgcctagg ccttgacggc cttccgccaa ttcgccctat agtgagtcgt 61 attacgtcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 181 gcccgcaccg aaacgccctt cccaacagtt gcgcagcctg aatggcgaat gggagcgccc 241 tgtagcggcc actcaaccct atctcggtct attcttttga tttataaggg attttgccga 301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 361 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 421 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 481 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 541 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 601 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 661 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 721 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 781 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 841 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 901 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 961 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 1021 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 1081 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 1141 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 1201 tgataaatct ggagccggtg agcgtggttc tcgcggtatc attgcagcac tggggccaga 1261 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 1321 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 1381 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 1441 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1501 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1561 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 1621 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 1681 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 1741 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 1801 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 1861 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 1921 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 1981 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2041 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 2101 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 2161 cctggccttt tgctggcctt ttgctcatta ggcaccccag gctttacccg aacgaccgag 2221 cgcagcgagt cagtgagcga ggaagcggag agcgcccaat acgcaaggaa acagctatga 2281 ccatgttaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcggaag 2341 gcccatgagg ccagttaatt aagaggtacc gaattcgcgg ccgcttctag atggccggct 2401 ggagccatcc gcagtttgaa aaaaccggcg acgtgtacca cgacggcgcc tgccccgaag 2461 tgaagcccgt ggacaacttc gactggtccc agtaccacgg caagtggtgg caggtggccg 2521 cttatcccga ccacatcacc aagtacggca agtgcggctg ggccgagtac acccccgagg 2581 gcaagagcgt gaaggtgtcc cggtacagcg tgatccacgg caaagagtac ttcagcgagg 2641 gcaccgccta ccctgtgggc gacagcaaga tcggcaagat ctaccacagc tacaccatcg 2701 gcggcgtgac ccaggtgggc gtgagcaacg tgctgtccac cgacaacaag aactacatca 2761 tcggctactt ttgcagatac gacgaggaca agaagggcca ctgggacgcc gtgtgggtgc 2821 tgtcccggtc catggtgctg accggcgagg ccaagaccgc cgtggagaac tacctgatcg 2881 gcagccccgt ggtggacagc cagaaactgg tgtacagcga cttctccgag gccgcctgca 2941 aagtgaacaa cagcaactgg tcccaccccc agttcgaaaa gaccggttaa tactagtagc 3001 ggccgctgca //