LOCUS Strep+FluA+Split 3622 bp DNA circular SYN 13-OCT-2009 DEFINITION Ligation of Strep+FluA+Split+FokA into pMA2_NgoMIV_SpeI ACCESSION Strep+FluA+Split KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3622) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+FluA+Split+FokA Fragment ends: NgoMIV and SpeI Fragment size: 1210 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2008..3219 /gene="ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2047..2568 /gene="FluA" /SECDrawAs="Gene" CDS 2575..2625 /gene="Split-Linker" /SECDrawAs="Gene" CDS 2629..3221 /gene="'Fok active'" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtgggag gtggccaagt accccagccc caacggcaag 2161 tatggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtccaga 2221 tacgacgtga tccacggcaa agaatacttc atggaaggca ccgcctaccc cgtgggcgac 2281 agcaagatcg gcaagatcta ccacagccgg accgtgggcg gctacaccag aaagaccgtg 2341 ttcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacagctg ccgctacgac 2401 gaggacaaga agggccactg ggaccacgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt cagcgaggcc gcctgcaaag tgaacaacac cggccgacca 2581 gcctgtaaga ttccaaatga cctgaagcag aaagttatga atcacaccgg caaatctgaa 2641 ctggaggaga aaaaatccga gctgcgccac aaactgaaat atgtgcctca cgagtatatc 2701 gaactgatcg agatcgcccg taatagtacc caagaccgta tcctggaaat gaaagtgatg 2761 gagttcttca tgaaagtcta tggctatcgt ggcaaacatc tgggtggtag ccgtaaacct 2821 gatggtgcca tttataccgt tggttccccg atcgattatg gcgttatcgt tgataccaaa 2881 gcctatagcg ggggttataa cctgccaatt ggtcaggctg atgagatgca gcgttatgtg 2941 aaagagaacc agactcgtaa caaacacatc aacccgaacg aatggtggaa agtgtatccg 3001 tcaagcgtta cagagttcaa attcctgttc gtgagcggcc attttaaagg caactataaa 3061 gcacagctga cccgtctgaa ccataaaacc aatagcaatg gcgccgttct gtcagtagaa 3121 gagctgctga ttggcggtga aatgatcaaa gccgggaccc tgacactgga agaagttcgc 3181 cgtaaattca acaatgggga gatcaatttt accggttaat actagtagcg gccgctgcag 3241 agctcatggc gcgcctaggc cttgacggcc ttccgccaat tcgccctata gtgagtcgta 3301 ttacgtcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 3361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 3421 cccgcaccga aacgcccttc ccaacagttg cgcagcctga atggcgaatg ggagcgccct 3481 gtagcggcca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 3541 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 3601 aatattaacg cttacaattt ag //