LOCUS Strep+Dig+Split+ 3652 bp DNA circular SYN 13-OCT-2009 DEFINITION Ligation of Strep+Dig+Split+FokI into pMA2_NgoMIV_SpeI ACCESSION Strep+Dig+Split+ KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3652) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+Dig+Split+FokI Fragment ends: NgoMIV and SpeI Fragment size: 1240 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2008..3249 /gene="ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2044..2599 /gene="'Dig'" /SECDrawAs="Gene" CDS 2605..2655 /gene="Split-Linker" /SECDrawAs="Gene" CDS 2659..3248 /gene="'Fok inaktiv'" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtggcag gtggccgctt atcccgacca catcaccaag 2161 tacggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtcccgg 2221 tacagcgtga tccacggcaa agagtacttc agcgagggca ccgcctaccc tgtgggcgac 2281 agcaagatcg gcaagatcta ccacagctac accatcggcg gcgtgaccca ggtgggcgtg 2341 agcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacttttg cagatacgac 2401 gaggacaaga agggccactg ggacgccgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt ctccgaggcc gcctgcaaag tgaacaacag caactggtcc 2581 cacccccagt tcgaaaagac cggccgacca gcctgtaaga ttccaaatga cctgaagcag 2641 aaagttatga atcacaccgg caaatctgaa ctggaggaga aaaaatccga gctgcgccac 2701 aaactgaaat atgtgcctca cgagtatatc gaactgatcg agatcgcccg taatagtacc 2761 caagaccgta tcctggaaat gaaagtgatg gagttcttca tgaaagtcta tggctatcgt 2821 ggcaaacatc tgggtggtag ccgtaaacca gcaggtgcca tttataccgt tggttccccg 2881 atcgattatg gcgttatcgt ggccacaaaa gcgtattctg gcggttataa tctgccgatt 2941 ggtcaggctg atgagatgga acgttatgtg gaagagaatc agacccgtaa caaacatctg 3001 aacccgaacg aatggtggaa agtgtatccg tcaagtgtca ccgagttcaa atttctgttc 3061 gtgagcggcc actttaaagg caactataaa gcccagctga ctcgtctgaa ccatatcacc 3121 aatagcaatg gggcagtgct gagtgttgag gaactgctga tcggtggaga aatgatcaaa 3181 gcaggcaccc tgactctgga agaagttcgc cgtaaattca acaatggcga gatcaatttt 3241 accggttaat actagtagcg gccgctgcag agctcatggc gcgcctaggc cttgacggcc 3301 ttccgccaat tcgccctata gtgagtcgta ttacgtcgcg ctcactggcc gtcgttttac 3361 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 3421 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga aacgcccttc ccaacagttg 3481 cgcagcctga atggcgaatg ggagcgccct gtagcggcca ctcaacccta tctcggtcta 3541 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3601 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ag //