LOCUS Strep+FluA+short 3583 bp DNA circular SYN 13-OCT-2009 DEFINITION Ligation of Strep+FluA+shortlinker+FokA into pMA2_NgoMIV_SpeI ACCESSION Strep+FluA+short KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3583) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+FluA+shortlinker+FokA Fragment ends: NgoMIV and SpeI Fragment size: 1171 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2008..3180 /gene="ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2047..2568 /gene="FluA" /SECDrawAs="Gene" CDS 2572..2586 /gene="'shortLinker26" /SECDrawAs="Gene" CDS 2590..3177 /gene="'FokA" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtgggag gtggccaagt accccagccc caacggcaag 2161 tatggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtccaga 2221 tacgacgtga tccacggcaa agaatacttc atggaaggca ccgcctaccc cgtgggcgac 2281 agcaagatcg gcaagatcta ccacagccgg accgtgggcg gctacaccag aaagaccgtg 2341 ttcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacagctg ccgctacgac 2401 gaggacaaga agggccactg ggaccacgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt cagcgaggcc gcctgcaaag tgaacaacac cggcggtggt 2581 tctggtaccg gcaaatctga actggaggag aaaaaatccg agctgcgcca caaactgaaa 2641 tatgtgcctc acgagtatat cgaactgatc gagatcgccc gtaatagtac ccaagaccgt 2701 atcctggaaa tgaaagtgat ggagttcttc atgaaagtct atggctatcg tggcaaacat 2761 ctgggtggta gccgtaaacc tgatggtgcc atttataccg ttggttcccc gatcgattat 2821 ggcgttatcg ttgataccaa agcctatagc gggggttata acctgccaat tggtcaggct 2881 gatgagatgc agcgttatgt gaaagagaac cagactcgta acaaacacat caacccgaac 2941 gaatggtgga aagtgtatcc gtcaagcgtt acagagttca aattcctgtt cgtgagcggc 3001 cattttaaag gcaactataa agcacagctg acccgtctga accataaaac caatagcaat 3061 ggcgccgttc tgtcagtaga agagctgctg attggcggtg aaatgatcaa agccgggacc 3121 ctgacactgg aagaagttcg ccgtaaattc aacaatgggg agatcaattt taccggttaa 3181 tactagtagc ggccgctgca gagctcatgg cgcgcctagg ccttgacggc cttccgccaa 3241 ttcgccctat agtgagtcgt attacgtcgc gctcactggc cgtcgtttta caacgtcgtg 3301 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 3361 gctggcgtaa tagcgaagag gcccgcaccg aaacgccctt cccaacagtt gcgcagcctg 3421 aatggcgaat gggagcgccc tgtagcggcc actcaaccct atctcggtct attcttttga 3481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3541 atttaacgcg aattttaaca aaatattaac gcttacaatt tag //