LOCUS       FluA+His+shortli        3577 bp    DNA     circular SYN 08-OCT-2009
DEFINITION  Ligation of FluA+His+shortlinker+FokA into pMA2_NgoMIV_SpeI
ACCESSION   FluA+His+shortli
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pMA2_NgoMIV_SpeI
               Fragment ends: SpeI and NgoMIV
               Fragment size: 2412
            Insert molecule:  FluA+His+shortlinker+FokA
               Fragment ends: NgoMIV and SpeI
               Fragment size: 1165
FEATURES             Location/Qualifiers
     CDS             129..989
                     /gene="AmpR"
                     /SECDrawAs="Gene"
     CDS             2017..3174
                     /gene="'ORF-1"
                     /SECDrawAs="Gene"
     CDS             2017..2034
                     /gene="His Tag"
                     /SECDrawAs="Gene"
     CDS             2041..2562
                     /gene="FluA"
                     /SECDrawAs="Gene"
     CDS             2566..2580
                     /gene="'shortLinker26"
                     /SECDrawAs="Gene"
     CDS             2584..3171
                     /gene="'FokA"
                     /SECDrawAs="Gene"
ORIGIN      
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
     1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
     1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
     1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
     2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
     2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
     2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
     2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
     2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
     2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
     2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
     2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
     2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggcgg tggttctggt
     2581 accggcaaat ctgaactgga ggagaaaaaa tccgagctgc gccacaaact gaaatatgtg
     2641 cctcacgagt atatcgaact gatcgagatc gcccgtaata gtacccaaga ccgtatcctg
     2701 gaaatgaaag tgatggagtt cttcatgaaa gtctatggct atcgtggcaa acatctgggt
     2761 ggtagccgta aacctgatgg tgccatttat accgttggtt ccccgatcga ttatggcgtt
     2821 atcgttgata ccaaagccta tagcgggggt tataacctgc caattggtca ggctgatgag
     2881 atgcagcgtt atgtgaaaga gaaccagact cgtaacaaac acatcaaccc gaacgaatgg
     2941 tggaaagtgt atccgtcaag cgttacagag ttcaaattcc tgttcgtgag cggccatttt
     3001 aaaggcaact ataaagcaca gctgacccgt ctgaaccata aaaccaatag caatggcgcc
     3061 gttctgtcag tagaagagct gctgattggc ggtgaaatga tcaaagccgg gaccctgaca
     3121 ctggaagaag ttcgccgtaa attcaacaat ggggagatca attttaccgg ttaatactag
     3181 tagcggccgc tgcagagctc atggcgcgcc taggccttga cggccttccg ccaattcgcc
     3241 ctatagtgag tcgtattacg tcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3301 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3361 gtaatagcga agaggcccgc accgaaacgc ccttcccaac agttgcgcag cctgaatggc
     3421 gaatgggagc gccctgtagc ggccactcaa ccctatctcg gtctattctt ttgatttata
     3481 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3541 cgcgaatttt aacaaaatat taacgcttac aatttag
//