LOCUS Longlinker+FokI_ 3051 bp DNA circular SYN 20-OCT-2009 DEFINITION Ligation of NgoMIV_FokI_PstI into Longlinker_AgeI_PstI_pMA ACCESSION Longlinker+FokI_ KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3051) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: Longlinker_AgeI_PstI_pMA Fragment ends: PstI and AgeI Fragment size: 2437 Insert molecule: NgoMIV_FokI_PstI Fragment ends: NgoMIV and PstI Fragment size: 614 FEATURES Location/Qualifiers CDS 27..72 /gene="LongLinker'" /SECDrawAs="Gene" CDS 27..665 /gene="ORF-1" /SECDrawAs="Gene" CDS 75..662 /gene="'FokI" /SECDrawAs="Gene" CDS complement (1686..2546) /gene="AmpR" /SECDrawAs="Gene" ORIGIN 1 gagctcgaat tcgcggccgc ttctagatgg ccggcggtgg ttctggtggt ggttctggtg 61 gtggttctgg taccggcaaa tctgaactgg aggagaaaaa atccgagctg cgccacaaac 121 tgaaatatgt gcctcacgag tatatcgaac tgatcgagat cgcccgtaat agtacccaag 181 accgtatcct ggaaatgaaa gtgatggagt tcttcatgaa agtctatggc tatcgtggca 241 aacatctggg tggtagccgt aaaccagcag gtgccattta taccgttggt tccccgatcg 301 attatggcgt tatcgtggcc acaaaagcgt attctggcgg ttataatctg ccgattggtc 361 aggctgatga gatggaacgt tatgtggaag agaatcagac ccgtaacaaa catctgaacc 421 cgaacgaatg gtggaaagtg tatccgtcaa gtgtcaccga gttcaaattt ctgttcgtga 481 gcggccactt taaaggcaac tataaagccc agctgactcg tctgaaccat atcaccaata 541 gcaatggggc agtgctgagt gttgaggaac tgctgatcgg tggagaaatg atcaaagcag 601 gcaccctgac tctggaagaa gttcgccgta aattcaacaa tggcgagatc aattttaccg 661 gttaatacta gtagcggccg cctgcagggt acctcttaat taactggcct catgggcctt 721 ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca 781 tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc 841 ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac 901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1801 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca 1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2641 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt 2701 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 2761 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtggc cgctacaggg 2821 cgctcccatt cgccattcag gctgcgcaac tgttgggaag ggcgtttcgg tgcgggcctc 2881 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 2941 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg acgtaatacg 3001 actcactata gggcgaattg gcggaaggcc gtcaaggcct aggcgcgcca t //